Background: Cryopreservation of farm animal embryos is nowadays a widely used routine technique. Success in cryopreservation of form animal depends on many factors including: freezing medium, types of cryoproctants, melting process, lipid content in ooplasm and oocyte quality It has been found that immature Oocytes with reduced reproductive performance or slaughtered at the end of their use are...

The Southampton In Vitro Fertilisation Programme was established in 1986. Since that time the programme has maintained a clinical pregnancy rate of over 25 per oocyte retrieval. Embryos in excess of requirements for fresh transfer have been cryopreserved using controlled rate freezing and Propanediol as a cryoprotectant. Review of the first 75 frozen embryo transfers in natural or clomid induce...

Autologous human oocyte cryopreservation allows storing woman’s eggs to be used later by the same donor when therapeutic or elective reasons induce women to postpone motherhood. In the case of non-medical motivation, this practice is termed social freezing. By shifting the awareness from a medical procedure to social relations, frozen oocytes are becoming polemic bio-objects, which pose new que...

Thanks to the recent advances in reproductive medicine, more and more young women with breast cancer may be offered the possibility of preserving their fertility. Fertility can be endangered by chemotherapy, by treatment duration and by patient's age at diagnosis. The currently available means to preserve a young woman's fertility are pharmacological protection with gonadotrophin-releasing horm...

OBJECTIVE To evaluate the efficacy of a new ultravitrification technique with a low concentration of cryoprotectants. DESIGN Ultravitrification research. SETTING Private assisted reproduction center. PATIENT(S) Oocytes donated voluntarily with the aim of research. INTERVENTION(S) Ultravitrification with different protocols of 100 mature oocytes and 100 immature oocytes divided in four g...

Ovarian hyperstimulation syndrome (OHSS) still remains a life-threatening complication of in vitro fertilization treatment (IVF), keeping patients and especially those, who previously experienced OHSS, from attempting infertility treatment and childbearing. The recent implementation of four new modalities: the GnRH antagonist protocol, GnRH agonist (GnRHa) triggering of ovulation, blastocyst tr...

Medical treatments at the beginning and end of human life are highly contested in public discourse. Our study reveals factors shaping acceptance social egg freezing (or oocyte cryopreservation) as an assisted reproduction technology (ART) general public. Based on theory moral contextualism literature medical ethics, we deduce potential influencing attitudes toward cryopreservation (for example,...

Is there a really need to validate oocyte vitrification technique in an ART laboratory before establishing it in daily practice? Validation of micromanipulationbased technique, in this case oocyte vitrification, is essential prior to enlarging its use to routine practice. Oocyte vitrification is a new worldwide used technique and legal recently in France. This micromanipulation needs to be perf...