Severe experimental infections with Cryptosporidium parvum have been reported in immunocompromised animals such as SCID mice (mice without functional T cells and B cells). In a C. parvum infection with 1 x 10(6) oocysts/mouse in SCID beige (SCIDbg) mice (SCID mice lacking functional NK cells), oocyst shedding was first demonstrated 18 days after infection. However, shedding was shown as early a...

Mosquito feeding assays are important in evaluations of malaria transmission-reducing interventions. The proportion of mosquitoes with midgut oocysts is commonly used as an outcome measure, but in natural low intensity infections the effect of oocyst non-rupture on mosquito infectivity is unclear. By identifying ruptured as well as intact oocysts, we show that in low intensity P. falciparum inf...

humoral immune response of vaccine-i (supermatent from sonicated sporulated oocyst), vaccine –ii (sediment from sonicated sporulated oocyst) and vaccine –iii (un-sonicated sporulated oocyst) against conccidiosis in chickens was determined by indirect haemagglutination (iha) test. iha antibody titre was significantly higher (p

Problem statement: Different methods of experimental infection of broiler chickens with Eimeria species have been described in the literature. These methods had not been compared and contrasted so as to contribute to the selection of the most appropriate model of coccidiosis in broiler chickens. Identifying such a model was important to speed up the screening of potential coccidiostatics. Appro...

OBJECTIVE To estimate the analytic sensitivity of microscopic detection of Toxoplasma gondii oocysts and the environmental loading of T gondii oocysts on the basis of prevalence of shedding by owned and unowned cats. DESIGN Cross-sectional survey. SAMPLE POPULATION 326 fecal samples from cats. PROCEDURES Fecal samples were collected from cat shelters, veterinary clinics, cat-owning househ...

Coccidiosis is one of the major parasitic diseases in commercial broiler industry. Probiotics can protect poultry against Eimeria infection. However, mechanisms are not fully known. Therefore, Lactobacillus plantarum P8 (P8) was used to investigate its anti-coccidial property and mechanism. Five hundred broilers were allocated five treatments: control diet (NC), + infection (IC), containing 1 ×...

The relationship between oocyst dose and lesion score was evaluated in trials involving five field isolates each of Eimeria acervulina, Eimeria maxima, and Eimeria tenella. Each trial included an uninfected, unmedicated treatment, and at least three treatments of unmedicated birds given different doses of oocysts from a single isolate. In four trials each with E. acervulina and E. tenella, and ...

Several in vitro surrogates have been developed as convenient, user-friendly alternatives to mouse infectivity assays for determining the viability of Cryptosporidium parvum oocysts. Such viability assays have been used increasingly to determine oocyst inactivation following treatment with chemical, physical, or environmental stresses. Defining the relationship between in vitro viability assays...

Accurate knowledge of the transport and deposition behavior for pathogenic Cryptosporidium parvum oocysts is needed to assess contamination and protect water resources. Experimental and modeling studies were undertaken to examine the roles of attachment, detachment, and straining on oocyst transport and retention. Saturated column studies were conducted using Ottawa aquifer sands (U.S. Silica, ...

The effect of age and dietary factors of Anopheles gambiae (Diptera: Culicidae) on the infectivity of natural Plasmodium falciparum parasites was studied. Mosquitoes of various ages (1-3, 4-7 and 8-11 day old) and those fed blood (either single or double meals) and sugar meals were experimentally co-infected with P. falciparum gametocytes obtained from different naturally infected human volunte...