In this investigation, a nano-sized protein-encapsulated polymeric micelle was prepared by self-assembling human serum albumin (HSA) as a model protein and degradable block copolymer methoxy poly(ethylene glycol)-poly(β-amino ester) (PEG-PAE) with piperidine and imidazole rings. From the zeta potential measurement, the protein-encapsulated polymeric micelle showed a pH-tuning charge conversion ...

BACKGROUND Experimental disruption of the telomere overhang induces a potent DNA damage response and is the target of newly emerging cancer therapeutics. Introduction of T-oligo, an eleven-base oligonucleotide homologous to the 3'-telomeric overhang, mimics telomere disruption and induces DNA damage responses through activation of p53, p73, p95/Nbs1, E2F1, pRb, and other DNA damage response pro...

The aim of this study was to examine whether the transcription factor ETS1 plays a critical role in the regulation of human decidualization. Decidual fibroblast cells were decidualized in vitro by treatment with medroxyprogesterone, estradiol (E(2)) and dibutyryl cyclic AMP or prostaglandin E(2) in the absence or presence of an ETS1 antisense oligonucleotide (oligo) that blocks the translation ...

The intradermal delivery of an antisense oligonucleotide was examined by iontophoresis. In this experiment, the antisense sequence of [(32)P]-labeled phosphodiester oligonucleotide ([(32)P]D-oligo, 18-mer) hybridizing to mouse interleukin 10 (IL-10) mRNA was used as a model D-oligo. In in vitro iontophoretic experiments, isolated hairless mouse skin was used with a horizontal diffusion cell. Th...

Human B- or T-cell lymphoma lines and primary murine lymphomas were treated with DNA oligonucleotides homologous to the telomere (TTAGGG repeat; "T-oligo"), either alone or in combination with standard, widely-used anticancer chemotherapeutic agents. T-oligo induces cell cycle arrest and apoptosis in cultured human or murine B or T-lymphoma cell lines and primary tumor cells, but exerts no dete...

Clathrate hydrates are supramolecular solids in which small, hydrophobic species are trapped inside a network of hydrogen-bonded water cages. While the best known examples are the natural gas hydrates, similar crystalline structures can also be formed from salts of tetra-n-butylammonium hydroxide (TBAOH). When TBAOH is reacted with medium to long chain carboxylic acids it produces surfactant co...

The poliovirus RNA-dependent RNA polymerase binds cooperatively to single-stranded RNA. We have determined the minimal RNA-binding site size of the poliovirus polymerase using binding titration with oligonucleotides of increasing length. A dramatic increase in affinity was observed when the length of the oligo(U) increased from 8 to 10 nucleotides (nt), arguing that the minimal size of RNA for ...

Intradiffusion coefficients of sodium alkylsulfonates [CH3-(CH2)n-1SO-3 Na+, CnSNa] (n = 5-9, 11) in mixtures with heavy water were measured by the PGSE-NMR technique at 25 degrees C. A slope change in the experimental trends permits the determination of the critical micelle concentration (CMC). In the micellar composition range, solubilized TMS molecules were used to determine the micelle intr...

Photo-controlled metal-ion (Zn(2+) and Cd(2+)) release in aqueous micelle solution (tris-HCl, pH = 7.4) has been described using 2-((2-mercaptophenylimino)methyl) phenol as ligand. It is found that both the ligand-Zn complex (1) and the ligand-Cd complex (2) are stable in micelle solution, and Zn(2+) (Cd(2+)) can be released from the complex with 365 nm light trigger. Accompanying the metal-ion...

2014 The effect of temperature on the aggregation behaviour of nonionic surfactants in aqueous solution has been investigated by means of transient fluorescence probing (intramicellar excimer formation by pyrene). For surfactants of the C12H25En type (E = ethyleneoxide group, n = 6, 8, 9) the number average aggregation number N of the micelle is nearly independent of the temperature T when T is...