Adenine nucleotides—adenosine monophosphate, diphosphate, and triphosphate—are of utmost importance to all living organisms, where they play a critical role in the energy metabolism are tied allosteric regulation various regulatory enzymes. Adenylate charge represents precise relationship between concentrations adenosine triphosphate indicates amount metabolic available an organism. The experim...

Studies on the cellular metabolism of endogenous nucleosides and structurally related analogs are facilitated by the thin-layer chromatographic (TLC) separation of nucleotides, Complex mixtures of ribosyl and deoxyribosyl nucleotides can be separated by ascending two-dimensional TLC on PEI-cellulose anion-exchange layers”“. Nucleoside mono-, diand triphosphates are first separated in one dimens...

Characterization of the structure and dynamics of nucleic acids by NMR benefits significantly from position specifically labeled nucleotides. Here an E. coli strain deficient in the transketolase gene (tktA) and grown on glucose that is labeled at different carbon sites is shown to facilitate cost-effective and large scale production of useful nucleotides. These nucleotides are site specificall...

To investigate the effects of dietary nucleotides on lipid metabolism and learning ability, male Sprague-Dawley rats were fed with a nucleotides-supplemented diet or a nucleotides-free diet for 5 weeks. The content of nucleotides in the diet was 1.0% and their composition resembled that in human milk. The content of phosphatidylcholine (PC) and the ratio of PC to phosphatidylethanolamine (PE) i...

Many of the building blocks life such as amino acids and nucleotides are chiral, i.e., different from their mirror image. Contemporary selects synthesizes only one two possible handednesses. In an abiotic environment, however, there usually equally many left- right-handed molecules. If homochirality was a prerequisite life, must have been physical or chemical circumstances that led to selection...

A high-performance liquid chromatographic method for the separation of nucleotide glycation reaction product(s) has been developed. The product(s) arising during in vitro glycation of a nucleotide (AMP, GMP or CMP) with a reducing sugar (ribose or glucose) were clearly resolved from the non-glycated constituents of the reaction mixture. The peak(s) of the glycated product(s) increased when an a...