نتایج جستجو برای: nucleic acid amplification techniques naat
تعداد نتایج: 1401179 فیلتر نتایج به سال:
LB-5. Hepatitis C Nucleic Acid Amplification Testing (NAAT) for Acute Case Detection: A Historical Protocol Repurposed to Combat a Current Outbreak Jessica Gentry, MA; Sara Blosser, PhD; Kathleen Backfish, BS, (M)CM; Michael Cross, BS; Marta Cruz, MT(ASCP); Edwin Hartle II,; Erica Vecchio, MS; Erika Chapman, MPH, CPH, CHES; Caitlin Conrad, BS; Joan Duwve, MD, MPH; Romeo Galang, MD, MPH; Daniel ...
Tuberculosis (TB) is one of the leading causes of adult death in the Asia-Pacific Region, including Indonesia. As an infectious disease caused by Mycobacterium tuberculosis (MTB), TB remains a major public health issue especially in developing nations due to the lack of adequate diagnostic testing facilities. Diagnosis of TB has entered an era of molecular detection that provides faster and mor...
BACKGROUND Two thirds of the world's new HIV infections are in sub-Saharan Africa. Acute HIV infection (AHI) is the time of virus acquisition until the appearance of HIV antibodies. Early HIV infection, which includes AHI, is the interval between virus acquisition and establishment of viral load set-point. This study aimed to detect acute and early HIV infections in a hyper-endemic setting. M...
Prevalence of HIV infection in the Philippines remains low. This may be partly due to reliance on passive reporting for surveillance. The algorithm for laboratory testing for HIV infection has become more stringent in the sense that the screening assays are repeated in the confirmatory centers, before Western Blot is performed. This has been due to the high rate of false positives before 2005. ...
BACKGROUND Nucleic acid amplification technologies (NAAT) are advancing our ability to make rapid molecular diagnoses in patients with serious culture negative infections. This is the first report of PCR coupled to electrospray ionization mass spectrometry use in the evaluation of complicated community acquired pneumonia in a pediatric patient. CASE PRESENTATION We present a case of culture n...
BACKGROUND The baboon (Papio hamadryas anubis) can be transcervically instrumented, facilitating studies of intrauterine contraception and reproductive tract infection. We sought to determine if the baboon could become infected with a single cervical inoculation of Chlamydia trachomatis. METHODS Ten female baboons were randomized and inoculated cervically with C. trachomatis serovar E (or buf...
Oligonucleotide-linked gold nanoparticle aggregates for enhanced sensitivity in lateral flow assays.
Lateral flow assays (LFAs) as rapid analytical techniques promise to be widely used in point-of-care (POC) diagnostics because of their affordability and simplicity. However, LFAs still suffer from low sensitivity in detection of various biomarkers, e.g., nucleic acids. In this study, we developed a simple and general one-step signal amplification strategy, which employed oligonucleotide-linked...
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