Relativistic density functional calculations were applied to study the separation behaviors of the Am(iii) ion from the Eu(iii) ion by diglycolamide (DGA) and nitrilotriacetamide (NTA) ligands in order to understand the difference in the separation mechanism of their reagents. The complexation reaction was modeled on the basis of previous experimental studies. The calculated energies based on s...

RAF kinases are key players in the MAPK signaling pathway and are important targets for personalized cancer therapy. RAF dimerization is part of the physiological activation mechanism, together with phosphorylation, and is known to convey resistance to RAF inhibitors. Herein, molecular dynamics simulations are used to show that phosphorylation of a key N-terminal acidic (NtA) motif facilitates ...

The German philosopher K.C.F. Krause (1781–1832) found deep conceptual parallels between his panentheistic system and the Indian philosophy of Ved?nta. This article critically examines Krause’s understanding Ved?nta popular Hindu religion. I argue that while was correct in viewing mystical doctrine as a precursor to own philosophy, he also frequently misled by unreliable translations secondary ...

The analytical technique DGT (Diffusive Gradients in Thin-films) is able to gain access a wealth of information by carefully interpreting accumulation data from passive samplers with different configurations (i.e. thicknesses its constituent layers). A set devices were simultaneously deployed solutions Ni and nitrilotriacetic acid (NTA) concentrations measure the availability these solutions. A...

Energy-resolved threshold collision-induced dissociation (TCID) of negatively-charged [amb5-H+M(II)+NTA-2H]− ternary complexes were studied for how they competitively dissociated into the products [amb5-3H+M(II)]− + NTA or [NTA-3H+M(II)]− amb5, where M = Zn Ni and is nitrilotriacetic acid. The contained one alternative metal binding-5 (amb5) heptapeptides with primary structures acetyl-Aa1-Aa2-...

Abstract It is well known that the Indian Ocean dipole (IOD) closely related to El Niño-Southern Oscillation (ENSO). In this study, it found spring–summer north tropical Atlantic (NTA) sea surface temperature (SST) anomalies can contribute development of IOD since mid-1980s as ENSO. After mid-1980s, an anticyclonic circulation over subtropical northeastern Pacific could be excited by cool NTA S...

BACKGROUND A versatile strategy for protein-surface coupling in biochips exploits the affinity for polyhistidine of the nitrilotriacetic acid (NTA) group loaded with Ni(II). Methods based on optical reflectivity measurements such as spectroscopic ellipsometry (SE) allow for label-free, non-invasive monitoring of molecule adsorption/desorption at surfaces. RESULTS This paper describes a SE stu...

BACKGROUND Extracellular vesicles (EV) released into the circulation after traumatic injury may influence complications. We thus evaluated the numbers of EV in plasma over 28 days after trauma and evaluated their pro-coagulant and inflammatory effects. METHODS AND FINDINGS 37 patients suffering trauma with an injury severity score >15 were studied along with 24 healthy controls. Plasma sample...

BACKGROUND Nanoparticle tracking analysis (NTA) and tunable resistive pulse sensing (TRPS) enable measurement of extracellular vesicles (EVs) in blood plasma but also measure other particles present in plasma. Complete isolation of EVs from similarly sized particles with full EV recovery is currently not possible due to limitations in existing isolation techniques. AIM This study aimed to eva...

BACKGROUND Topping is an important cultivating measure for flue-cured tobacco, and many genes had been found to be differentially expressed in response to topping. But it is still unclear how these genes are regulated. MiRNAs play a critical role in post-transcriptional gene regulation, so we sequenced two sRNA libraries from tobacco roots before and after topping, with a view to exploring tran...