Resistance of Nosema ceranae to different exposure conditions has been evaluated by using Sytox green and DAPI (4',6-diamidino-2-phenylindole) to test spore viability. High thermotolerance at 60 and 35 degrees C and resistance to desiccation were observed. However, a significant decrease in viability after freezing and a rapid degeneration of spores maintained at 4 degrees C were also detected.

In this work, we proposed a novel electrochemical immunosensor for sensitive detection of the total protein of Nosema bombycis based on Fe3O4 nanoparticles (Fe3O4NPs) as catalyst to electrocatalyze the reduction of methylene blue (MB) with the aid of Fe3O4NPs-DNA dendrimers for the signal amplification.

Although purely vertically transmitted parasites are predicted to cause low pathogenicity in their hosts, the effects of such parasites on host fitness under stressful environmental conditions have not previously been assessed. Here, we investigate the effects of Nosema granulosis, a vertically transmitted, microsporidian parasite of the brackish water amphipod Gammarus duebeni, on host growth ...

A microsporidium was isolated from larvae of Spodoptera litura (Fabricius) collected from Tamil Nadu, India. This microsporidian species is monomorphic, disporous and develops in direct contact with the cytoplasm of the host cell. The nuclear configuration of merogonic and sporogonic stages was diplokaryotic. The merogonic proliferative stage was unusual that normal development with 1, 2 and 4 ...

nosema apis is an obligate intracellular parasite belonging to phylum microsporidia. this para site is known as main causative agent of nosemosis in honeybees especially in the european honeybee (apis mellifera). nosemosis can cause queen supersedure, reduction of honey yield and dwindling the population of honeybees. a total number of 54 apiaries were randomly sampled from april to july 2011 a...

Two microsporidians are known to infect honey bees: Nosema apis and Nosema ceranae. Whereas population genetics data for the latter have been released in the last few years, such information is still missing for N. apis. Here we analyze the patterns of nucleotide polymorphism at three single-copy loci (PTP2, PTP3 and RPB1) in a collection of Apis mellifera isolates from all over the world, natu...

Light microscopic examination of feces from a human immunodeficiency virus-positive patient with chronic diarrhea, anorexia, and lethargy revealed the presence of numerous refractile bodies resembling microsporidian spores. They were subsequently identified as belonging to the genus Nosema on the basis of their ultrastructural characteristics. However, the microsporidia were enclosed within str...

iv INTRODUCTION AND LITERATURE REVIEW 1 Potential for Control 1 Nomenclature and Life History 3 Effect of Microsporidian Parasites on 8 Vectored Disease Host Specificity of Other Microsporida .... 9 The Predators Tested 15 Purpose of the Present Study 21 MATERIALS AND METHODS 22 Maintenance of Infected Mosquitoes 22 Collection and Maintenance of Predators .... 24 Standard Feeding Procedure 26 T...