نتایج جستجو برای: northern blotting

تعداد نتایج: 148215  

Journal: :iranian journal of biotechnology 2015
khadijeh razavi sasan mohsenzadeh mohammad ali malboobi tahmineh lohrasebi farhad ghavami

aeluropus lagopoides is a salt and drought tolerant grass from poaceae family, distributed widely in arid regions. there is almost no information about the genetics or genome of this close relative of wheat that stands harsh conditions of deserts. differential display amplified fragment length polymorphism (dd-aflp) led to the improvement of a non-radioactive method for which many parameters we...

Journal: :Physiological genomics 2002
Yihua Zhao Benjamin P C Chen Hui Miao Suli Yuan Yi-Shuan Li Yingli Hu David M Rocke Shu Chien

Statistical methods for identifying differentially expressed genes from microarray data are evolving. We developed a test for the statistical significance of differential expression as a function of time. When applied to microarray data obtained from endothelial cells exposed to shearing for different durations, the new multi-group test (G-test) identified three times as many genes as the one-w...

Journal: :The American journal of tropical medicine and hygiene 2006
Allen C Cheng Mathew O'brien Kevin Freeman Gary Lum Bart J Currie

Melioidosis is caused by the saprophytic organism Burkholderia pseudomallei. The use of the indirect hemagglutination assay (IHA) has found widespread use in areas endemic for this disease. Using this assay, we explored the serologic profile of 275 patients with culture-confirmed melioidosis in the Northern Territory of Australia. Based on a threshold titer of 1:40, the sensitivity of the IHA o...

Journal: :The Journal of general virology 2007
Chris Cowled Lorna Melville Richard Weir Susan Walsh Alex Hyatt Rosey Van Driel Steven Davis Aneta Gubala David Boyle

Middle Point orbivirus (MPOV) was isolated in 1998 from a healthy cow pastured at Beatrice Hill farm, Middle Point (formerly Coastal Plains Research Station), 50 km east of Darwin in Australia's Northern Territory. The isolate could not be identified by using conventional serological tests, and electron microscopy indicated that it belongs to the family Reoviridae, genus Orbivirus. Genetic sequ...

2001
H. van der Laan

We have used the Westerbork Northern Sky Survey (WENSS) to define a sample of 47 low redshift (z <∼ 0.4) giant radio galaxies. This is the largest sample yet of such radio sources originating from a single survey. We present radio maps of the newly discovered giants and optical images and spectra of their host galaxies. We present some of their properties and discuss the selection effects. We i...

Journal: :Developmental Immunology 1990
D. Dunon J. Salomonsen K. Skjødt J. Kaufman B. A. Imhof

Expression of chicken MHC class I (B-F) antigens during ontogeny was determined by binding of anticlass I antibody and appearance of B-F transcripts by Northern blotting in chicken organs during embryogenesis until 2 weeks after hatching. MHC class I transcripts first become detectable in day 6.5 of embryogenesis. B-F cell-surface expression first becomes detectable in hemopoietic organs by day...

2014
David Warrilow Daniel Watterson Roy A. Hall Steven S. Davis Richard Weir Nina Kurucz Peter Whelan Richard Allcock Sonja Hall-Mendelin Caitlin A. O’Brien Jody Hobson-Peters

Here we describe Casuarina virus (CASV), a new virus in the family Mesoniviridae. This is the first report of a mesonivirus in Australia, which extends the geographical range of this virus family to 3 continents. The virus was isolated in 2010 from Coquillettidia xanthogaster mosquitoes during surveillance in the suburbs of Darwin, the capital of the Northern Territory. Cryo-electron microscopy...

Journal: :Circulation research 1992
H L Kanter J E Saffitz E C Beyer

Electrical propagation in the normal heart occurs via intercellular transfer of current at gap junctions. Alterations in intercellular coupling in the diseased heart are critical in the pathogenesis of reentrant ventricular arrhythmias. Until recently only a single gap junction protein was known to couple cardiac myocytes. We have now identified and sequenced two additional distinct gap junctio...

Journal: :BioTechniques 1998
P Igaz G Fejér C Szalai S Tóth A Falus

The transcription factor junB belongs to the jun family of protooncogenes. The appearance of junB mRNA in hepatic cells is an extremely early and sensitive marker of the action of proinflammatory cytokines including interleukin-6. In this study, a competitive reverse transcription (RT)-PCR assay has been developed that is suitable for the quantitative determination of junB mRNA expression. This...

Journal: :Blood pressure 2010
Charlotte Lepoutre-Lussey Pierre-François Plouin Olivier Steichen

Northern Africans constitute one of the largest immigrant groups in France; however, limited data are available on their cardiovascular risk factors. We carried out a retrospective cross-sectional study in a French hypertension unit, comparing 719 patients born in Northern Africa to 3558 controls born in Europe, individually matched for age and sex. Using a Bonferroni adjusted alpha-level=0.001...

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