With the aim of investigating glucose-mediated glucose disposal (glucose effectiveness [GE]) in 15 (3 female and 12 male subjects) insulin-resistant normoglycemic relatives of patients with type 2 diabetes (DM2), and 15 age-, sex-, and BMI-matched control subjects without a family history of DM2, we performed 2 studies: 1) a 5-h euglycemic near-normoinsulinemic pancreatic clamp with somatostati...

Neutrophil nonoxidative defense mechanisms include several low molecular weight antimicrobial peptides. We have isolated a proline-arginine-rich antibacterial peptide from porcine neutrophils. This cationic, low molecular weight peptide has a very high degree of identity (97%) to a bactericidal peptide, PR-39, that has been found in the porcine small intestine. Isolation of the same antimicrobi...

To determine if mechanisms other than the generation of toxic oxygen intermediates are active against intracellular pathogens, oxidatively deficient mouse L cells and monocyte-derived macrophages from patients with chronic granulomatous disease were stimulated with soluble lymphocyte products. Despite no enhancement in oxidative activity, these cells displayed effective microbistatic activity a...

Heterophils are the predominate granulated leukocyte in the acute inflammatory response in gallinaceous birds. Heterophils are highly phagocytic and are capable of a broad spectrum of antimicrobial activity. They accumulate in inflamed tissue, causing tissue damage and forming heterophil granulomas that are morphologically similar to inflammatory lesions in reptiles. The avian heterophil lacks ...

Chronic granulomatous disease (CGD) is a primary immunodeficiency disorder caused by inherited defects in the nicotinamide adenine dinucleotide phosphate oxidase complex. The neutrophils of patient with CGD can ingest bacteria normally, but the oxidative processes that lead to superoxide anion formation, hydrogen peroxide production, nonoxidative pathway activation, and bacterial killing are im...

Abstract Herein, we report a new simple and easy-to-use approach for the characterization of protein oligomerization based on fluorescence resonance energy transfer (FRET) capillary electrophoresis with LED-induced detection. The FRET pair consisted quantum dots (QDs) used as an emission tunable donor (emission wavelength 450 nm) cyanine dye (Cy3), providing optimal optical properties acceptor....

Two novel ruthenium complexes belonging to the Ru(II)(DAD)(Cym) (DAD = diazadienyl) (Cym cymene) compound family are introduced as promising precursors. Their chemical nature, potential for vapor deposition (CVD), and possibly atomic layer (ALD) demonstrated. The development of nonoxidative CVD processes yielding high-quality Ru thin films is realized. Chemical analyses exercised that vitiate d...