Material and methods The study was conducted in the Venereal Diseases Department, St. Bartholomew's Hospital, London. The diagnosis of nongonococcal urethritis was based on the presence of a sexually acquired urethral discharge, containing abundant polymorphonuclear leucocytes, shown not to be due to gonorrhoea by negative findings in Gramstained smears and by negative results to cultures. Case...

The prevalence and clinical features of Chlamydia trachomatis infection were studied in men with nongonococcal urethritis (NGU), in their female sexual partners, and in other women. Of 92 patients with NGU, 36 (39%) were chlamydia-positive; although all had symptoms of urethritis, fewer than half had evident discharge. Clinical features of chlamydia-positive and chlamydia-negative NGU were indi...

The percentage of gonococcal isolates in King County, Washington, requiring citrulline and uracil (CU auxotype) increased from 1.6% in 1986 to 16.5% in 1997. Among men, urethral infection with the CU auxotype (n=93), in comparison with infection by other auxotypes (n=1211), was associated with coexisting chlamydial infection, younger age, heterosexual contact, and fewer new recent partners (P<....

To evaluate whether bacterial vaginosis predicts the acquisition of sexually transmitted diseases (STDs), we studied 255 nonpregnant female subjects aged 15-30 who reported recent sexual contact with a male partner in whom either gonococcal or chlamydial urethritis or nongonococcal urethritis was diagnosed. Compared to subjects with normal vaginal flora, subjects with bacterial vaginosis were m...

In an earlier study, Ford and McCandlish (1969) isolated TRic agents, by the yolk-sac method, from fifteen of 133 urethral scrapings of men with nongonococcal urethritis. Gordon and Quan (1965) had previously found that TRIC agents would propagate in irradiated McCoy cells and could be readily demonstrated by the resulting inclusions which stained purple with iodine because of their glycogen co...

A reverse passive hemagglutination test that utilizes human erythrocytes coated with antibody to gonococci was developed to distinguish differences among 11 strains of Neisseria gonorrhoeae. Different rabbits were immunized with each strain of gonococcus. Antibody was purified by passing antiserum over an immunoadsorbent column containing homologous cell walls trapped in a cross-linked polyacry...