T H E posterior segment isolated from definitive streak or head process blastoderms by a transverse cut 0 • 5-0 • 2 mm. posterior to Hensen's node seems to be a suitable experimental object for the study of differentiation. Its limited prospective significance and potency in chorioallantoic transplantation or in vitro compared with the versatile node has been the subject of several studies (Hun...

the present work developed an efficient plant regeneration and propagation system via direct organogenesis for morinda officinalis how., a valuable endangered medicinal plant. the effects of explant types on shoot induction, plant growth regulators on shoot proliferationand elongation and the subsequent rooting ability of shoots were examined. among the various types of explant, direct shoot pr...

In the present study, synthetic seed production of medicinally important plant Enicostema axillare through nodal and root explants were attempted. plantlets germinated seeds using in vitro explants. Synthetic prepared from 3% Na+ 100mm calcium chloride was optimum concentration for uniform compact synseed formation. Alginate beads containing derived successfully entrapped with sodium alginate h...

In general, antimicrobial agents are often used in micropropagation techniques to obtain contaminant free clones. The objective of the present study was evaluate effects bavistin and cefotaxime on producing plants Ruellia tuberosa cultured MS supplemented with phytohormones. Field grown nodal explants regenerate entire via direct organogenesis. Among decontaminants tested, fungicide along highe...

An efficient in-vitro micropropagation protocol has been developed for Berberis chitria (Lindl.) - a rare medicinal plant from Himachal Pradesh, India using seeds and nodal explants. The effects of different concentrations BAP GA3 seed germination was investigated consequently 6.0 mg/l + 0.0 reported to be excellent germination. Callus induction leaf explants aseptically grown seedling were als...

Background: Optimization of co-cultivation parameters during Agrobacterium-mediated transformation to Euphorbia tirucalli evaluated were bacterial density, infection period, acetosyringone (AS) concentration and temperature. Optimized resulted in high efficiency 3 fold increase at transient GUS expression .The optimized conditions Agrobacterium tumefaciens growth phase A600nm 0.17, period 30 mi...

present study was conducted to consider the effects of different concentrations of 2,4-d and ba on somatic embryogenesis induction, development and maturation of three strawberry cultivars (kurdistan, parose and camarosa). for this purpose, leaf blade, nodal, petiole, stamen and flower bud calli were cultured on murashige and skoog (ms) medium supplemented with 2, 4 dichlorophenoxyacetic acid (...

in vitro propagation of plant has played a very important role in rapidmultiplication of cultivars with desirable traits and production of healthy and disease-free plants. in the present investigation, the objectives were to optimize the micropropagation of rose hybrid ‘black baccarat’ cultivar. in proliferation step, the nodal segments (1.5 cm) was cultured on both liquid and solid media (ms, ...

Crataeva religiosa G. Forst. var. nurvala (Buch.-Ham.) Hook. F. & Thomson (syn. Crataeva nurvala Buch.-Ham.), a fast growing medicinal tree species, has been successfully micropropagated under in vitro conditions. Direct multiple shoots were regenerated from nodal explants on Murashige and Skoog’s (MS) medium, solidified by agar and supplemented with 1 mg/L 6-benzyl amino purine (BAP) and 0.5 m...

A protocol for rapid multiplication of Adhatoda vasica has been developed through nodal explants from field grown mature plants. The maximum number of shoots, i.e., 7.75 +/- 0.392 differentiated from split nodal halves on MS medium supplemented with BA (10.0 mg/l) during 4 weeks of culture. Maximum number of shoots formed per explant increased to ca. 30 within 6 weeks of subculture on medium co...