Abstract. Nitro-aromatic compounds (NACs) were measured hourly at a rural site in China during wintertime to monitor the changes due local and regional impacts of biomass burning (BB). Concurrent continuous measurements concentrations 16 NACs gas particle phases performed with time-of-flight chemical ionization mass spectrometer (CIMS) equipped Filter Inlet for Gases AEROsols (FIGAERO) unit usi...

(-)-Epigallocatechin-3-gallate (EGCG) has exhibited been studied for lung cancer inhibitory activity in vitro and in animal models, but it is rapidly methylated and inactivated by catechol-O-methyltransferase (COMT). Entacapone and tolcapone, COMT inhibitors, are used to mitigate the symptoms of Parkinson's disease. We investigated the synergistic effects of entacapone/tolcapone and EGCG agains...

A manganese-dependent 3,4-dihydroxyphenylactate 2,3-dioxygenase from Arthrobacter globiformis strain CM-2 (MndD) cloned in Escherichia coli has been purified to homogeneity. Sedimentation equilibrium analysis indicates an alpha 4 homotetrameric holoenzyme structure (4 x 38,861 Da). Steady-state kinetic analysis of MndD with a variety of substrates and inhibitors yields very similar relative rat...

(1) The brains of 17-day-old quaking and jimpy mice were compared with those of the corresponding normal phenotypes. The concentrations of cerebroside and sulphatide were found to be markedly lower in the affected mutants, while the relative amounts of ceramide and free fatty acid appeared normal. (2) The concentration of cerebroside glactosidase was not significantly abnormal in the jimpy mice...

for AICHE 2004 [15C12] Advances in Biocatalysis and Protein Engineering Tailoring Toluene para-Monooxygenase of Ralstonia pickettii PKO1 for Regiospecific Oxidation of Aromatics Using Active Site Engineering A. Fishman, Y. Tao, W. E. Bentley, and T. K. Wood University of Connecticut, Storrs, CT University of Maryland, College Park, MD Oxygenases are promising biocatalysts for performing selecti...

Phase II metabolites, or conjugates, are highly polar, non-volatile and thermolabile compounds that need to be analysed in biological matrices such as urine, bile and plasma. Liquid chromatography (LC) and capillary electrophoresis (CE) are proven techniques for the analysis of compounds of this kind, but the standard detectors in LC and CE lack sensitivity and especially specificity. Coupling ...

Uptake, depuration, and metabolism ofp-nitroanisole (PNA) and p-nitrophenol (PNP) were investigated in Halim eda, Padina, and Porolithon species, all of which are calcareous marine algae found in tropical waters . The algae were exposed to filtered seawater solutions of either PNA or PNP in a static system for 24 hr (uptake period), then placed in clean water and allowed to release absorbed che...

The relative proportions of arylsulphatase A and B were determined by the method of BAUM, DODGSON and SPENCER (1959) in brains of various animal species and it was found that there was a considerable variation in the concentration of these two enzymes. Arylsulphatase A and B of various animal species including rat, man, monkey, sheep and chicken were partially separated using zinc acetate fract...

Accumulation of sulfolipids associated with markedly elevated levels of glycolipid sulfotransferase activities was previously demonstrated in human renal cell carcinoma cells. To explore the regulation mechanisms of sulfoglycolipid synthesis in renal cancer, effects of various growth factors on the metabolic enzymes of sulfoglycolipids were investigated by using a human renal cell carcinoma cel...

Methods have been developed for the quantitative assay of cytochrome oxidase, esterase, and 11 acid hydrolases in rat-spleen homogenates. These methods seem to be applicable also to other lymphoid tissues. Preliminary studies, extended to nine of the acid hydrolases, indicate that these enzymes occur in partly latent and sedimentable form and that they can be unmasked and rendered soluble by so...