Rhodococcus sp. strain M8 is a nitrile-degrading bacterium isolated from acrylonitrile-contaminated sites. This strain produces the enzymes for sequential nitrile degradation, cobalt-type nitrile hydratase, and amidase in large amounts. Its draft genome sequence, announced here, has an estimated size of 6.3 Mbp.

Alcaligenes sp. MTCC 10674 has a bienzymatic system for the hydrolysis of nitriles. The nitrile hydratase and amidase have been purified simultaneously to homogeneity using a combination of (NH)4SO4 precipitation, ion exchange chromatography and gel permeation chromatography. Nitrile hydratase and amidase have molecular weight of 47 and 114 kDa, respectively and exist as heterodimer. Optimum te...

Abstract Nitrilium betaine 1,3-dipoles are ubiquitous reagents in organic chemistry, with applications ranging from natural product synthesis to materials science. Given the high reactivity of these zwitterionic motifs, they invariably generated situ a suitable precursor, prior use. This short review summarises recent progress development modern approaches towards formation 1,3-dipoles, and the...

the present study was conducted to examine the effect of using latex and nitrile examination gloves on hand dexterity.  a  sample  consisting  of  30  university  students  from  urmia  university  of  medical  sciences participated in this study in 2013. the participants conducted purdue pegboard test in three experimental conditions: bare hand (control), with latex gloves and with nitrile glo...

Rhodococcus rhodochrous NCIMB 11216 produced nitrile hydratase (320 nkat mg of protein(-1)) and amidase activity (38.4 nkat mg of protein(-1)) when grown on a medium containing propionitrile. These enzymes were able to hydrolyze nitrile groups of both granular polyacrylonitriles (PAN) and acrylic fibers. Nitrile groups of PAN40 (molecular mass, 40 kDa) and PAN190 (molecular mass, 190 kDa) were ...

E,Z-5-Aroylmethylene-3-benzyl-4-oxo-2-thioxo-1,3-thiazolidines (3a-c) react with 4-methoxy and 4-chlorophenylnitrile oxides (4a and b) in pyridine solution to afford one or more of the following compounds: Z-3, Z-2,4-dioxo analogues 5 and 3,6-diaryl1,4,2,5-dioxadiazines (6a-b). The interconversion route is discussed and the structures of all of the synthesised compounds are proven by microanaly...

The chemical industry has recently begun to look at enzymes as novel catalysts for many organic syntheses. Nitrile-hydrolyzing enzymes such as nitrile hydratase and nitrilase have great potential as catalysts in processing organic chemical because they can convert nitriles to the corresponding higher-value amides or acids. Recently, the use of bacterial nitrile hydratase for industrial producti...

Based on monitoring the γ process (the lowest temperature-relaxation) in polyacrylonitrile (PAN) by dynamic dielectric spectroscopy, new evidence for the formation of a charge transfer complex between bromine dopants and nitrile groups is presented. The experimental work is carried out on PAN and nitrile polymerized PAN with and without bromine doping and the effects of these factors on the γ p...

The distribution of phenylacetaldoxime-degrading and pyridine-3-aldoxime-degrading ability was examined with intact cells of 975 microorganisms, including 45 genera of bacteria, 11 genera of actinomyces, 22 genera of yeasts, and 37 genera of fungi, by monitoring the decrease of the aldoximes by high-pressure liquid chromatography. The abilities were found to be widely distributed in bacteria, a...