This medium, dispensed in 100-mi amounts in 250-ml Erlenmeyer flasks, was inoculated with an agar block containing L colonies which had been growing 5 days following 15 passages on a staphylococcal L form maintenance agar (Marston, J. Infectious Diseases, 108, 75-84, 1961). Slight turbidity and a granular sediment developed in the liquid medium in 4 days at 37 C and serial transfer of the sedim...