نتایج جستجو برای: nihayah indicates his utmost religious fanaticism

تعداد نتایج: 380906  

Journal: :The Journal of General Physiology 2001
Michael Gordon Alan Finkelstein

In the presence of a low pH environment, the channel-forming T domain of diphtheria toxin undergoes a conformational change that allows for both its own insertion into planar lipid bilayers and the translocation of the toxin's catalytic domain across them. Given that the T domain contributes only three transmembrane segments, and the channel is permeable to ions as large as glucosamine(+) and N...

Journal: :Biotechnology progress 2005
Dong Soo Hwang Youngsoo Gim Hyung Joon Cha

Mussel adhesive proteins, including the 20-plus variants of foot protein type 3 (fp-3), have been suggested as potential environmentally friendly adhesives for use in aqueous conditions and in medicine. Here we report the novel production of a recombinant Mytilus galloprovincialis foot protein type 3 variant A (Mgfp-3A) fused with a hexahistidine affinity ligand in Escherichia coli and its appr...

Journal: :Analytical chemistry 2006
Farid Khan Mingyue He Michael J Taussig

There is a particular need in protein analysis and purification for specific, functional, and generic methods of protein immobilization on solid supports. Here we describe a double-hexahistidine (His6) tag sequence, comprising two hexahistidines separated by an 11-amino acid spacer, which shows at least 1 order of magnitude stronger binding to Ni-NTA-modified surfaces than a conventional single...

2016
Ernest C. So Gunnar N. Schroeder Danielle Carson Corinna Mattheis Aurélie Mousnier Malgorzata Broncel Edward W. Tate Gad Frankel

Legionella pneumophila, the causative agent of Legionnaire's disease, uses its type IV secretion system to translocate over 300 effector proteins into host cells. These effectors subvert host cell signaling pathways to ensure bacterial proliferation. Despite their importance for pathogenesis, the roles of most of the effectors are yet to be characterized. Key to understanding the function of ef...

Journal: :Gene 2006
Zakee L Sabree Veit Bergendahl Mark R Liles Richard R Burgess Robert M Goodman Jo Handelsman

Acidobacterium capsulatum is an acid-tolerant, encapsulated, Gram-negative member of the ubiquitous, but poorly understood Acidobacteria phylum. Little is known about the genetics and regulatory mechanisms of A. capsulatum. To begin to address this gap, we identified the gene encoding the A. capsulatum major sigma factor, rpoD, which encodes a 597-amino acid protein with a predicted sequence hi...

Journal: :The Journal of biological chemistry 2006
William A Anong Tahlia L Weis Philip S Low

The principal bridge connecting the erythrocyte membrane to the spectrin-based skeleton is established by band 3 and ankyrin; mutations leading to reduced bridge formation or increased bridge rupture result in morphological and mechanical abnormalities. Because membrane mechanical properties are determined in part by the protein interactions that stabilize the membrane, we have evaluated the ra...

2017
Keith M. Macaulay Geraldine A. Heath Alessio Ciulli Alex M. Murphy Chris Abell John P. Carr Alison G. Smith

The important plant hormone salicylic acid (SA; 2-hydroxybenzoic acid) regulates several key plant responses including, most notably, defence against pathogens. A key enzyme for SA biosynthesis is isochorismate synthase (ICS), which converts chorismate into isochorismate, and for which there are two genes in Arabidopsis thaliana One (AtICS1) has been shown to be required for increased SA biosyn...

Journal: :Molecules and cells 2001
K H Kim K S Kim S E Choi J K Kim J H Park J H Lee Y Kang

Many recombinant proteins are produced as a fusion protein tagging hexahistidine, which has been used for studying their biological function both in vitro and in vivo. Unexpectedly, we observed activation of BALB/c mouse splenocytes when treated with hexahistidine-tagged recombinant proteins. This activation was hexahistidine-specific since the anti-pentahistidine antibody completely neutralize...

Journal: :Bioscience, biotechnology, and biochemistry 2010
Xiaoyan Yang Takayoshi Kinoshita Masaki Gouda Koichi Yokota Toshiji Tada

Fyn-related kinase (Frk) was first identified using human breast cancer cells. It shares 51% identity with c-Src. Like all members of the Src family, Frk is thought to cause several cancers via dysregulations in signal transduction from cell-surface receptors. The excess activity of Frk on beta-cells has a crucial role in type-I diabetes. A silent mutation at Ile229 conferred a bacterial expres...

Journal: :Biological chemistry 2012
Maryta Sztukowska Florian Veillard Barbara Potempa Matthew Bogyo Jan J Enghild Ida B Thogersen Ky-Anh Nguyen Jan Potempa

RgpA and Kgp gingipains are non-covalent complexes of endoprotease catalytic and hemagglutinin-adhesin domains on the surface of Porphyromonas gingivalis. A motif conserved in each domain has been suggested to function as an oligomerization motif. We tested this hypothesis by mutating motif residues to hexahistidine or insertion of hexahistidine tag to disrupt the motif within the Kgp catalytic...

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