Previous studies of the physiology of an aromatic-deficient mutant strain of Neurospora crassa, Y7655a (1, 2), have led to the following conclusions: (a) The biosynthesis of the aromatic rings of phenylalanine, tyrosine, tryptophan and p-aminobenzoic acid proceeds in Neurospora as it does in Escherichia coli (3) via 5-dehydroshikimic acid and shikimic acid. (b) Protocatechuic acid, the major co...

In most eukaryotes, chromosome rearrangements are recognized cytologically by visibly altered chromosome structure, or genetically by altered linkage relations. The first translocations in Neurospora were detected and verified using these methods (McClintock 1945, Houlahan et al. 1949). However, characteristics of Neurospora provide an easier method that depends on ascospore pigmentation, and t...

Previous observations of morphological, reproductive and genetic variation have suggested that Neurospora discreta, as presently circumscribed, might represent a diverse complex of multiple species. To investigate this hypothesis we examined the phylogenetic relationships among 73 fungal strains traditionally identified as N. discreta. Strains were chosen from across the morphological, ecologic...

Four arginaseless mutants of Neurospora crassa have been isolated. All carry mutations which lie at a single locus, aga, on linkage group VIIR. A study of aga strains shows the arginase reaction to be the major, perhaps the only, route of arginine consumption in Neurospora other than protein synthesis. Ornithine-delta-transaminase, the second enzyme of the arginine catabolic pathway, is present...

We have constructed a genomic library of Neurospora crassa DNA in a cosmid vector that contains the dominant selectable marker for benomyl resistance. The library is arranged to permit the rapid cloning of Neurospora genes by either sib-selection or colony-hybridization protocols. Detailed procedures for the uses of the library are described. By use of these procedures, a modest number of unrel...

We have determined the DNA sequence of the 4070 base pair mitochondrial plasmid from the Labelle-1b strain of Neurospora intermedia. Analysis of the sequence revealed that the plasmid contains a long open reading frame (ORF) that could encode a protein of up to 1151 amino acids. Codon usage in the long ORF shows no clear relationship to Neurospora mitochondrial genes, nuclear genes, nor to the ...

The Grainy head (GRH) family of transcription factors are crucial for the development and repair of epidermal barriers in all animals in which they have been studied. This is a high-level functional conservation, as the known structural and enzymatic genes regulated by GRH proteins differ between species depending on the type of epidermal barrier being formed. Interestingly, members of the CP2 ...

Conidial anastomosis tubes (CATs) can be recognized in 73 species of filamentous fungi covering 21 genera, and develop in culture and in host-pathogen systems. They have been shown to be morphologically and physiologically distinct from germ tubes in Colletotrichum and Neurospora, and under separate genetic control in Neurospora. CATs are short, thin, usually unbranched and arise from conidia o...

To align the four cyanogen bromide peptides of Neurospora tyrosinase whose amino acid sequences were reported in the preceding paper, suitable methionine-containing overlap peptides were isolated. The required peptides were obtained by tryptic, peptic, and thermolytic digestion of the unmodified protein and of the maleylated derivative. From the partial sequence information of these peptides an...

Neurospora crassa is a filamentous fungus and a model organism for the study of the molecular basis of circadian clocks. Rhythmic sporulation of this organism is maintained for prolonged periods in the dark with a periodicity of approximately 22 h. Three genes; frequency, white collar-1 and white collar-2, and their associated kinases and phosphatases, are key components of the circadian clock ...