نتایج جستجو برای: nested rt pcr
تعداد نتایج: 222624 فیلتر نتایج به سال:
شناسایی مراکزتکثیر آلوده به ویروس بیماری نکروز عفونی بافت های خونساز(ihn) در پنج استان عمده تولید کننده تخم چشم زده و بچه ماهی قزل آلای رنگین کمان کشور شامل مازندران، چهارمحال و بختیاری ، کهگیلویه و بویر احمد ، لرستان و فارس با استفاده از تکنیک های ifatو nested-rt-pcr و مقایسه این دو تکنیک با یکدیگر مورد مطالعه قرار گرفته است. بافت های کلیه، کبد و طحال بچه ماهی قزل آلای رنگین کمان طی شرایط استر...
In order to identify predominant circulating types of infectious bronchitis(IB) virus in the period of 1999-2004 in Iran, samples(trachea, lung, kidney) from 150 various flocks showing respiratory signs suspected to be related to IB infection were inoculated to SPF eggs. Then allantoic fluids studied by RT-PCR and Nested PCR using group and type specific primers to probing Massachusset, 793/B...
BACKGROUND Dengue fever results from infection with one or more of four different serotypes of dengue virus (DENV). Despite the widespread nature of this infection, available molecular diagnostics have significant limitations. The aim of this study was to develop a multiplex, real-time, reverse transcriptase-PCR (rRT-PCR) for the detection, quantitation, and serotyping of dengue viruses in a si...
Pathogenic bacterium Renibacterium salmoninarum causes kidney disease with high mortality rate and considerable economic losses in salmonid farming. Thus, application of fast and sensitive method for R. salmoninarum diagnosis is of great importance. This paper describes detection of R. salmoninarum in brook trout tissue with gross clinical signs of disease by nested RT–PCR. Determination of par...
There are high levels of co-incidence of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) in porcine tissue. This study established a duplex nested reverse transcriptase polymerase chain reaction (RT-PCR) method that targets the genomic RNA of type 2 PRRSV and the mRNA of PCV2 in infected tissues. The method amplified discriminative bands of 347 b...
Three multiplex hemi-nested RT-PCR assays were developed to detect simultaneously 12 RNA respiratory viruses: influenza viruses A, B and C, human respiratory syncytial virus (hRSV), human metapneumovirus (hMPV), parainfluenza virus types 1-4 (PIV-1, -2, -3 and -4), human coronavirus OC43 and 229E (HCoV) and rhinovirus (hRV). An internal amplification control was included in one of the RT-PCR as...
برونشیت عفونی طیور (IB) به وسیله کروناویروس ها ایجاد می‌شود. برونشیت یکی از عوامل مهم خسارت اقتصادی در طیور بوده که سبب درگیر شدن بیماریهای تنفسی ، نفریت و کاهش تولید و کیفیت تخم مرغ می‌شود. ویروس برونشیت دارای سروتیپ‌های متعددی بوده که ایمنی متقاطع در مقابل همدیگر ایجاد نمی‌کنند. هدف از این مطالعه ردیابی سروتیپ‌های ویروس برونشیت عفونی طیور در فارم‌های گوشتی استان گیلان دارای علائم تنفسی بوده ا...
We have developed a single-tube nested real-time PCR (STN-RT PCR) assay using the repetitive, transposon-like element IS1111 as the DNA target to facilitate early diagnosis of acute Q fever. The use of our proposed diagnostic procedures, including IgM detection by serology and the STN-RT PCR assay, significantly increased the diagnostic sensitivity for Q fever to 78%, compared to 29% when serol...
background: influenza a viruses bearing all known ha and na subtypes have been isolated from avian hosts: but only the a/h1n1,a/h2n2 and a/h3n2 influenza subtypes have been associated with widespread epidemics in humans until now .while influenza b detected only in humans. subtype h5 infection in humans occurred for the first time during 1997-1998 in hong kong, then china in 2003, vietnam and...
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