Segmentation of myelinated nerve fiber cross sections (MNFC) from a sequence of microscopic cross-sectional images, which can be used to construct the three-dimensional (3D) structure of nerve fiber, is important for evaluating neurological disorders and nerve regeneration. However, most existing segmentation methods do not take the spatial relationships between inter-frame MNFCs into account,s...

To our knowledge, this is the first demonstration of in vivo depth-resolved birefringence measurements of the human retinal nerve fiber layer (RNFL) by use of polarization-sensitive optical coherence tomography (PS-OCT). Because glaucoma causes nerve fiber layer damage, which may cause loss of retinal birefringence, PS-OCT is a potentially useful technique for the early detection of glaucoma. W...

PURPOSE We defined the quantitative changes in corneal epithelial nerve fibers during mouse development. METHODS Corneal whole mounts from 36 mice were immunostained with a specific anti-β-III tubulin antibody to label nerve fibers. Epithelial nerve fibers in the whole cornea were scanned and imaged using deconvolution microscopy. Subbasal nerve fiber and nerve terminal densities were calcula...

BACKGROUND The loss of peripheral nerve fiber is evident in chronic painful diabetic neuropathy. However, the correlation between peripheral fiber loss and the genesis of pain is unclear. Using the streptozotocin-induced diabetic rat model and focusing on free nerve endings, we attempted to investigate the peripheral changes that elicit pain syndromes in diabetes. METHODS Diabetes was induced...

PURPOSE To evaluate if posterior capsule opacification (PCO) would alter the analysis of nerve fiber layer using the scanning laser polarimetry (GDx). METHODS Pseudophakic non-glaucomatosous eyes (37 patients) underwent nerve fiber layer analysis using GDx before and after posterior capsulotomy with Nd:YAG Laser. RESULTS In five eyes, GDx did not obtain acceptable images using the device's ...

This paper describes an image acquisition and processing chain toward the analysis of images of thin histologic sections of the cerebral human cortex. We are particularly interested in the spatial distributions of nerve fibers, the inhomogeneities of which indicate architectonic and functional changes between different cortical areas. We therefore focus on the development of a specific optical ...

Since branching region of an active nerve fiber is an abrupt widening of the structure, two concepts emerge: first, the stimulating current must be sufficient to raise the outgrowing fibers above the thresh¬old, and secondly, the stimulating current will be divided in proportion to the characteristic admittance of the branches. On the other hand, blocking of the nerve impulse in this region is ...

Since branching region of an active nerve fiber is an abrupt widening of the structure, two concepts emerge: first, the stimulating current must be sufficient to raise the outgrowing fibers above the thresh¬old, and secondly, the stimulating current will be divided in proportion to the characteristic admittance of the branches. On the other hand, blocking of the nerve impulse in this region is ...

A histochemical staining technique for cholinesterase is reported for the study of corneal nerves in whole-thickness rabbit cornea. The technique is relatively simple, yields reproducible results, allows good definition of the nerve fiber pattern, and provides the investigator, using a binocular microscope, with the advantage of three-dimensional study. When tissue is stained for specific choli...

purpose : to compare the ability of scanning laser polarimetry (gdx vcc), and optical coherence tomography (oct) to discriminate eyes with primary open angle glaucoma (poag) from normal eyes and to assess the relationship between their parameters methods : eighty-seven glaucomatous eyes of 87 patients and 25 normal eyes were enrolled. the mean age of patients and normal group were 54.96±18.34 a...