Maastrichtian to Ypresian-dated sediments from the 12 Frères borehole. (Fresco, southwestern Côte d'Ivoire), are here investigated for paleoenvironmental and paleogeographic significance. For this study, preparation of palynological slides in laboratory is done three steps.: (1) sampling physical treatment, (2) chemical attacks (HCl 10%, HF 40%) dissolve mineral matter preserve organic containe...