Cinchona officinalis is an important species from the Andean cloud forest that has a low regeneration rate in natural populations. In vitro of C. been successfully established but somaclonal variation was not evaluated. The pathway and number subcultures on were evaluated using six ISSR primers amplified 58 loci Inter Simple Sequence Repeats (ISSR). A dendrogram based Jaccard´s genetic distance...

Abstract Female and male plants of difficult-to-root species Leucadendron elimense subsp. were investigated for rooting potential with three hormones: indole-3-acetic acid (IAA), 1-naphthaleneacetic (NAA), indole-3-butyric (IBA) at different concentrations (2000 ppm, 4000 6000 ppm). The experiment was made under essential misting, bottom heat, a naturally ventilated greenhouse. After 18 weeks t...

During exponential phase, the tobacco (Nicotiana tabacum) cell line cv Virginia Bright Italia-0 divides axially to produce linear cell files of distinct polarity. This axial division is controlled by exogenous auxin. We used exponential tobacco cv Virginia Bright Italia-0 cells to dissect early auxin signaling, with cell division and cell elongation as physiological markers. Experiments with 1-...

Auxin is one type of phytohormones that plays important roles in nearly all aspects of plant growth and developmental processes. The glycosylation of auxins is considered to be an essential mechanism to control the level of active auxins. Thus, the identification of auxin glycosyltransferases is of great significance for further understanding the auxin regulation. In this study, we biochemicall...

The hormone auxin is transported in plants through the combined actions of diffusion and specific auxin influx and efflux carriers. In contrast to auxin efflux, for which there are well documented inhibitors, understanding the developmental roles of carrier-mediated auxin influx has been hampered by the absence of specific competitive inhibitors. However, several molecules that inhibit auxin in...

Licorice plants, Glycyrrhiza glabra, G. uralensis, and G. inflata, were investigated for callus induction using Murashige and Skoog (MS) medium combined with auxins and cytokinins. After 4 weeks of culture, 33-100% of leaf or stem explants formed calli. Maximum of shoot induction from callus cultures was achieved by G. inflata stem explants cultured on MS medium supplemented with 1 mg/l alpha-n...

This paper describes a protocol for the efficient vegetative propagation of Cleome rosea by somatic embryogenesis. Leaf and stem explants from nursery-grown seedlings of C. rosea were cultivated on Murashige and Skoog (MS) medium supplemented with indole-3-acetic acid (IAA), α-naphthaleneacetic acid (NAA), 4-amino-3,5,6trichloropicolinic acid (picloram) or 2,4-dichlorophenoxyacetic acid (2,4-D)...

Free amino acid constituents were determined of grape stem and Phylloxera leaf gall callus in tissue culture. Fast, medium and slow growing single cell clones of, respectively, stem and gall origins were grown on a mineral salt-sucrose medium supplemented with coconut milk and alpha-naphthaleneacetic acid. Stem and gall clones showed qualitative similarities and quantitative variations in the a...

The study compared the effects of 1.0 × MS medium containing various concentrations of α-naphthaleneacetic acid (NAA), indole-3-acetic acid (IAA), and indole-3-butyric acid (IBA), alone or in combination with gibberellic acid (GA3) in micropropagation of three potato (Solanum tuberosum L.) cultivars Pasinler, Granola, and Caspar using binodal stem cuttings. The results testified improved regene...