During organogenesis, cell fate specification and patterning are regulated by signaling centers, specialized clusters of morphogen-expressing cells. In many organs, initiation of development is marked by bud formation, but the cellular mechanisms involved are ill defined. Here, we use the mouse incisor tooth as a model to study budding morphogenesis. We show that a group of nonproliferative epi...

Background Endometrial cancer is the most common gynecologic malignancy in the United States, with over 40,000 cases diagnosed each year, typically in the postmenopausal women. 25% of cases affect premenopausal women. 14% of endometrial cancers are diagnosed in women younger than 45 years old. 5% of these tumors are diagnosed in women younger than 40 years old. In this talk the learning objecti...

OBJECTIVES This study investigated prognostic significance of tumor budding in early-stage cervical cancer (ESCC) following radical surgery and its contribution to improve the stratification of patients with recurrence risk. METHODS The archival medical records and H&E-stained slides of 643 patients with IA2-IIA stage cervical cancer who underwent radical surgery were retrospectively reviewed...

BELL, G., and L. M. WOLFE. 1985. Sexual and asexual reproduction in a natural population of Hydra pseudoligactis. Can. J. Zool. 63: 851 -856. Populations of Hydra pseudoligactis were censussed during 1980198 1 in a lake and a small pond in southern Quebec. Both natural and artificial substrates (glass slides) were used. Population density rose during the early part of the sedson to a maximum in...

The maintenance of an appropriate number of telomere repeats by telomerase is essential for proper chromosome protection. The action of telomerase at the telomere terminus is regulated by opposing activities that either recruit/activate the enzyme at shorter telomeres or inhibit it at longer ones, thus achieving a stable average telomere length. To elucidate the mechanistic details of telomeras...

The lymphatic system is indispensable for the collection and cycling of tissue-extravasated fluids, macromolecules and immune cells into the bloodstream. Different mechanisms, including sprouting, ballooning and budding of lymphatic endothelial cells from the cardinal vein, have been proposed for lymphatic vessel formation during mammalial embryogenesis. Hägerling et al (2013) now provide a cel...

In budding yeast, two classes of post-Golgi secretory vesicles carrying different sets of cargoes typified by Bgl2p and invertase are delivered to the plasma membrane for secretion. The exocyst is implicated in tethering these vesicles to the daughter cell membrane for exocytosis. In this study, we report that mutations in the exocyst component Exo70p predominantly block secretion of the Bgl2p ...

In fission yeast and multicellular organisms, centromere-proximal regions of chromosomes are heterochromatic, containing proteins that silence gene expression. In contrast, the relationship between heterochromatin proteins and kinetochore function in the budding yeast Saccharomyces cerevisiae remains largely unexplored. Here we report that the yeast heterochromatin protein Sir1 is a component o...

Harvest time and storage had significant effects on nutrient quality Volatile Organic Compounds (VOCs) of alfalfa. The objective this study was to use headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC-MS) analyze alfalfa at different harvest periods (budding stage, early blooming, full blooming), for 0 d, 90 180 270 360 the dynamic changes in VOCs nutritiona...