A surface plasmon resonance imaging-based Ni(2+)-iminodiacetic acid-coated gold chip system was developed to enable specific detection of a hexahistidine-tagged recombinant protein in crude extracts or in column chromatography fractions. This system is especially advantageous for high-throughput analysis of multiple proteins.

Fluorescent probes are essential for the exploration of protein function, detection of molecular interactions, and conformational changes. The nitrilotriacetic acid derivatives of different chromophores were successfully used for site-selective noncovalent fluorescence labeling of histidine-tagged proteins. All of them, however, suffer from the same drawback--loss of the fluorescence upon bindi...

Affinity tags have become powerful tools from basic biological research to structural and functional proteomics. They were widely used to facilitate the purification and detection of proteins of interest, as well as the separation of protein complexes. Here, we mainly discuss the benefits and drawbacks of several affinity or epitope tags frequently used, including hexahistidine tag, FLAG tag, S...

this article studies sir walter scott’s use of the family-as-nation trope in ivanhoe and anne of geierstein. the importance of this trope in his figuration of nationhood is discerned by carrying out a close reading of the texts and placing them in their historical context, which primarily consists of a comparison with edmund burke and maria edgeworth. consequently, his position is revealed as b...

Another mention of what could be a cataract operation figures in the Bible, in the Book of Tobit (around 600 B.C.). Following instructions from the archangel Raphaël, Tobias applies the gall of a fish to his father’s blind eyes, «He applied the remedy which irritated his eyes. And he rubbed the corner of his eyes with both hands. Then he saw his son, threw his arms around his neck and wept as h...

The link between asthma and cows' milk is familiar to many young asthma sufferers and their parents. I first became aware of the connection through my cousin's experiences with his four-year-old son. Since infancy, my cousin's son has experienced severe asthma attacks and has been hospitalized twice for asthma-related pneumonia. When his asthma attacks become more frequent or more severe, my co...

A nurse wishes her son and family had received it after his cancer diagnosis.