نتایج جستجو برای: mtt 3 45 dimethylthiazolyl 25 diphenyl tetrazolium bromide assay
تعداد نتایج: 2332703 فیلتر نتایج به سال:
Unavoidable exposure to radiosensitive normal tissues around cancerous tumor during 9 the radiotherapy can cause side effects such as self-limited acute toxicities, mild chronic symptoms, 10 or severe organ dysfunction. Nevertheless, clinical use of currently available radiation protective 11 agents is limited because of their generic cytotoxicity. A study on radiation protective effect of 12 d...
Background: In respect to leishmaniasis prevalence in different regions of Iran and world and also several side effects of pentavalent antimony for treatment of the disease, using of medicinal plants have been recently taken into attention. The aim of this study was to evaluate the effect of garlic plant extract on L. major amastigotes in vivo and in vitro by means of colorimetric assay. Materi...
The present work evaluated the cytotoxicity of piplartine {5,6-dihydro-1-[1-oxo-3-(3,4,5-trimethoxyphenyl)-trans-2-propenyl]-2(1H)pyridinone} and piperine {1-[5-(1,3)-benzodioxol-5-yl)-1-oxo-2,4-pentadienyl]piperidine}, components obtained from Piper species. The substances were tested for their cytotoxicity on the brine shrimp lethality assay, sea urchin eggs development, 3-(4,5-dimethyl-2-thi...
Abstract This study aimed to evaluate, in vitro and vivo, the biocompatibility of experimental methacrylate-based endodontic sealers containing α-tricalcium phosphate (α-TCP) or nanostructured hydroxyapatite (HAp). Experimental dual-cure with addition α-TCP HAp, at 10%wt were formulated compared AH Plus (AHP). Cell viability was assessed by 3-(4,5-dimethyl-thiazoyl)-2,5-diphenyl-tetrazolium bro...
Five new oxygenated pimarane diterpenes, named scopararanes C-G (1-5) were isolated from the culture of a marine sediment-derived fungus Eutypella scoparia FS26 obtained from the South China Sea. The structures of these compounds were established on the basis of extensive spectroscopic analysis. The absolute configurations of compounds 1-5, were determined by CD spectroscopic analysis and compa...
The yellow tetrazolium salt 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) is widely used to determine cell viability in cell proliferation and cytotoxic assays. MTT is reduced by metabolically active cells to form an insoluble purple formazan product that is quantifiable by spectrophotometry. It is the most common and direct assay for cell viability. However, in this pres...
Introduction Afzelin is a glycosyloxyflavone that kaempferol attached to an alpha-L-rhamnosyl residue at position 3 via glycosidic linkage. It has role as plant metabolite, antimicrobial (Antibacterial and antifungal) agent anti-inflammatory agent. glycosyloxyflavone, trihydroxyflavone monosaccharide derivative. In this work, we determined the anticholinergic, antiepileptic, antidiabetic, anti-...
Abstract. Afililla Z, Suwanti LT, Mufasirin, Koerniawan HP, Suyono EA, Budiman A, Siregar UJ. 2022. The immunomodulatory effect of Glagah consortium microalgae polysaccharides based on splenocyte proliferation and cytokine production in vitro. Biodiversitas 23: 5205-5209. Indonesia is known as a maritime country that has various organisms, including microalgae. Some have many bioactivities, suc...
The colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was standardized for direct detection of rifampin-resistant Mycobacterium tuberculosis in sputum samples. The sensitivity and specificity of the direct MTT assay matched those of the standard indirect susceptibility assay on 7H10 medium, and interpretable results were obtained for 98.5% of the samples wit...
Objectives. Chemotherapy is considered to be essential in the treatment of patients with colorectal cancer (CRC), but drug resistance reduces its efficacy. Many advanced CRC eventually show 5-fluorouracil (5-FU) therapy. Synergistic and potentiating effects combination therapy, using herbal chemical drugs, can improve patients’ response. Zerumbone (ZER), which derived from ginger, has been stud...
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