Isotope-coded affinity tags (ICAT) is a labeling technique that provides insights into quantitative molecular changes. In this paper, we propose a new protocol to identify and analyze ICAT labeled peak pairs in high-resolution LC-MS data. Our major contributions are: (1) we use isotope distance constraint, ICAT distance constraint, and LC-span constraint to identify ICAT labeled peak pairs and ...

Objective: Multiple Sclerosis (MS) is one of the prevalent autoimmune diseases that affects the central nervous system. It is a chronic neurological disorder that progresses to physical inability and cognitive abnormalities restricting the individual’s ability to function independently. The problems of MS patients significantly affect their psychological well-being. Considering the problems of ...

Analyzing blood serum for opioids, cocaine and metabolites is a routine task in forensic laboratories. The most commonly used methods involve several manual or partly-automated sample preparation steps such as protein precipitation, solid phase extraction, evaporation and derivatization followed by GC/MS or LC/MS determination. In this study a comprehensively automated method is compared with a...

The methods of molecular biology allow for analyzing the methylation pattern in the whole genome and in particular genes. We differentiate methylated sequences from unmethylated ones by means of cutting the genomic template with methylation-sensitive restriction enzymes or by sodium bisulfite DNA modification. Chemical modification precedes most quantitative and qualitative PCR techniques: MS-P...

States and districts have embraced the goals of standards-based reform, but they have interpreted the rather broad objectives in their own unique ways. Merely setting the mark may not be enough to ensure adequate progress in our nation’s schools, Ms. Goertz warns. Comments Copyright Phi Delta Kappa International. Reprinted from Phi Delta Kappan, Volume 83, Issue 1, September 2001, pages 62-66. ...

1 Challenges in justifying the ICT expenditure The discussion commenced by discussing matters affecting the decisions to spend on ICT by government. Dr Kereteletswe indicated that we need to be in a position to justify to the Ministry of Finance and Development Planning the ICT expenditure. The chairman further added that currently the entire world is recovering from the economic melt-down henc...

The author hypothesized that multiple sclerosis (MS) is a humoral autoimmune disease, caused by faulty interplay between myelin-specific, dimeric IgE, specifically competing non-IgE antibodies and IgE-triggered degranulating mast cells. The principal fault was believed to be insufficient quantity of protective, specific non-IgE antibodies. Also conjectured was the possibility of an unexpected a...

The laser ablation (LA) method is an effective technique for quantitative analysis. In the present work, a new LA system was developed for the high-sensitivity analysis of metal materials using inductively coupled plasma mass spectrometry (ICP-MS). This system consists of a high-frequency Q-switched laser and 2 scanning mirrors for scanning the ablation spot in an adequately large area of the s...

Cloud computing is a significant alternative in today’s educational perspective. The technology gives the students and teachers the opportunity to quickly access various application platforms and resources through the web pages on-demand. Unfortunately, not all educational institutions often have an ability to take full advantages of the newest information technology. The paper analyzes possibi...

In this work, we present the Text Conditioned Auxiliary Classifier Generative Adversarial Network, (TAC-GAN) a text to image Generative Adversarial Network (GAN) for synthesizing images from their text descriptions. Former approaches have tried to condition the generative process on the textual data; but allying it to the usage of class information, known to diversify the generated samples and ...