Background Whole ovary cryopreservation has been proved to be a feasible technique in fertility preservation. The normal ovarian cortex contains a large population of primordial follicles, this characteristic make them far more tolerant to cryoinjury. Researchers have used different cryoprotectants and various techniques to improve cryopreservation. It is plausible that inclusion of an antioxid...

Background The aim of this study is to examine the effects of cranberry extract prescription in sensitive days of pregnancy on development and histomorphometric changes in liver of mouse embryo. MaterialsAndMethods A total of 24 pregnant mice were divided into equal 4 groups. The control group received no injection, while sham group was injected with saline and experimental groups were given cr...

Background: Embryo vitrification was effectively used for assisted reproductive techniques. Despite the undeniable benefits of vitrification, cooling and warming stress, and cytotoxicity of cryoprotectant may affect the DNA methylation that have an important role in gene activation and silencing. In the present study effects of 2-cell embryo vitrification on DNA methylation in hatched blastocys...

Background: It is assumed that adult stem/progenitor cells are responsible for cycling remodeling of the uterus endometrium throughout the reproductive life of the female. This study aimed to identify and localize stem/progenitor cells in the mice uterus using immunohistochemistry. Materials and Methods: 6-8 weeks old virgin female NMRI mice were submitted to the vaginal smear examination to de...

Background: Evaluation of maturation genes expression and their pattern during in vitro culture of vitrified preantral follicles is an essential and could promote either vitrification procedure or culture condition. Materials and Methods: Preantral follicles (with 100- 130 μm diameter) isolated mechanically from 12-14 days old NMRI mice ovaries and divided into vitrification and control groups....

Background Sample preparation is the first step in the evaluation of tissue samples. In this research tried to using two types of formalin and Bouin fixative ovarian tissue morphogenesis be evaluated practical. MaterialsAndMethods Ovaries of 6-8 week-old Naval Medical Research Institute female mice were randomly divided into 3 groups: Ι. Ovaries that immediately fixed in Bouin’s fixative; II. O...

Background This research was conducted to assess preantral follicle development in fibrin alginate matrix following ovarian tissue vitrification MaterialsAndMethods Ovaries of 13-day-old NMRI female mice were removed and placed in control and vitrification groups. Vitrification group ovaries were transferred in media containing ethylene glycol, dimethyl sulphoxide, and sucrose then were plunged...

Hepcidin is an innate immune element which decreases the iron absorption from diet and iron releasing from macrophage cell. In contrast to the chemical iron chelators, there has been limited effort applied to the specific use of hepcidin as a new drug for decreasing the iron overload. Hepcidin is produced in different biological systems. For instance, E-coli is used for human hepcidin expressio...

Introduction: The aim of this study was evaluate the ability of notochord to induce neural induction and/or differentiation of mouse embryonic stem cell to neuron and motor neuron, respectively. Methods: In order to produce embryoid bodies, ES cells line Royan B1 were grown in suspension in the absence of LIF for 4 days. EBs were divided into 4 groups. EBs in group 1 & 2 were further cultur...