BACKGROUND The present study aimed to evaluate the efficacy of the hyaluronic acid (HA) binding assay in the selection of motile spermatozoa with normal morphology at high magnification (8400x). METHODS A total of 16592 prepared spermatozoa were selected and classified into two groups: Group I, spermatozoa which presented their head attached to an HA substance (HA-bound sperm), and Group II, ...

BACKGROUND Timed and frequent intercourse around the time of female ovulation is recommended to improve conception. Although a significant number of articles have examined how the length of abstinence affects these semen analysis, the effects of frequent (daily) ejaculation has not been rigorously studied. METHODS Twenty normal men were recruited for daily ejaculation over 14 consecutive days...

Sperm with reduced motility has reduced fertilizing capacity. If low-motile Duroc sperm is mixed with high-motile sperm, the fertilizing capacity of that dose is equal to semen doses exclusively containing high-motile Duroc sperm. With doses that only contained low-motile sperm, the number of total born piglets per litter dropped by minimum 0.5. Duroc boars were selected on the basis of the per...

Background: Obesity increases the likelihood of various diseases, particularly heart disease, type 2 diabetes, obstructive sleep apnea, certain types of cancer and infertility and dub fertility. Some studies have associated the body mass index (BMI) with reproductive parameters in men, showing that increased BMI is related to poor semen quality, decreased sperm concentration, decreased normal-m...

in 30–50% of sub-fertile couples the male partner has poor semen quality, either because of low sperm count, poorly motile sperm or sperm with abnormal size and shape (morphology). This may result from inherent genetic factors, poor pre-natal testicular development or adult exposure to gonadotoxic agents. There are few proven therapies to improve semen quality. Therefore, the number and quality...

One in six couples of reproductive age worldwide are affected at least once by some form of infertility. In vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) are widely-available assisted reproductive technologies (ART). The identification and isolation of the most-motile sperm with DNA integrity are essential to IVF and ICSI, ultimately affecting treatment consequences and ...

The present study aimed to verify the caprine semen characteristics during dry and rainy seasons in the Brazilian Northeast, and the influence of these seasons on cooled semen. Seminal volume, concentration, percentage of motile cells, vigor and spermatic morphology, as well as biochemical profile (fructose, citric acid, P, Ca 2+ , Mg, total proteins and phospholipase A2 activity) were analyzed...

Background: Fertilization and fecundation obviously are the phenomenon in which several factors are involved. Sperm membranous proteins are a series of these important elements. CD9, an inner acrosomal membrane protein, is one of the tetraspanin members that involved in sperm-egg fusion. CD52, is a GPI anchor protein which is expressed in the epididymal cells and during passage of sperms throug...

OBJECTIVE To evaluate DNA fragmentation in spermatozoa with normal morphological appearance. DESIGN Prospective study. SETTING Academic tertiary center. PATIENT(S) Fertile, subfertile, and infertile men were studied. INTERVENTION(S) Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-fluorescein nick-end labeling assay and morphology assessment by phase contrast in ...