introduction: production of monoclonal antibody against alkaline phosphatase for application in immunohistochemical and immunocytochemical techniques such as alkaline phosphatase anti-alkaline phosphatase (apaap) method. material and methods: in this investigation female balb/c mice were immunized by several injections of alkaline phosphatase and the antibody titer in their sera were measured a...

background: monoclonal antibody technology allows identification of amastigote-specific antigen in human tissue biopsies and search for a suitable vaccine candidate, which may induce a long lasting immunity.   objectives : production and partial characterization of seven monoclonal antibodies against leishmania tropica promstigotes.   methods: seven murine monoclonal antibodies of the igg isoty...

conclusions the cell lines obtained in this work secreted potent, stable and specific anti-bv mabs, which were suitable for the development of herpes b virus diagnosis reagents. background herpes b virus (bv) is a zoonotic disease caused by double-stranded enveloped dna virus with cercopithecidae as its natural host. the mortality rate of infected people could be up to 70% with fatal encephalit...

Cryo-EM reveals the molecular basis of SARS-CoV-2 cross-neutralization by monoclonal antibody 47D11.

immunoglobulin e is one of the five classes of immonoglobulins that plays an important role in allergic diseases. production of monoclonal antibodies by a single clonotype against different epitopes of immunoglobulin e has high priority in development of diagnostic kits. in this study, an attempt was made to produce monoclonal antibodies against human immunoglobulin e. balb/c mice were immunize...

Introduction: Monoclonal gammopathy of renal significance (MGRS) is described as a hematologic condition characterized by nephrotoxicmonoclonal proteins produced non-malignant B-cell or plasma cell clone. Nevertheless, MGRS can cause serious lesions, leading to high morbidity. In C3 glomerulonephritis, monoclonal protein damage indirectly. Acting an autoantibody, the cannot be detected in kidne...

Background: Monoclonal antibodies have been employed extensively for the identification of Leishmania species, development of diagnostic tests and in the characterization of defined leishmanial antigens. Objectives: Identification and characterization of  Leishmania spp. directly from cutaneous lesions of infected individuals. Methods: An immunoperoxidase test (Avidin-Biotin technique) using mo...

Radiolabeled monoclonal antibodies, have created new innovations in diagnosis, research and therapy of disease in last 2 decades. One of the serious limitation of applications of radiolabeled antibodies in vivo is relatively low target to background activity. Various strategies have been proposed to solve this problem including pre-targeting methods that was suggested in 1989. Regarding i...

Background: Different methods have been used for characterization of Leishmania promastigotes. Monoclonal antibodies are useful in characterization of   Leishmania spp . both amastogotes and promastigotes. Objective: Comparing the characterization of   Leishmania spp. promastigotes with immunoperoxidase test (Avidin-Biotin) techniques and an indirect immunofluorescent assay (IFA).   Methods: Ap...

Background and Objective: Anti-CK5/6 monoclonal antibodies have an established role in breast disease diagnosis. Anti-CK5 monoclonal antibodies have recently become commercially available. There has been growing interest in the staining characteristics of anti-CK5 and its potential diagnostic role in place of anti-CK5/6. We aim to compare and contrast the staining characteristi...