نتایج جستجو برای: mlck

تعداد نتایج: 574  

2015
XIAOWEN CHENG YUFENG WAN YUANHONG XU QING ZHOU YUAN WANG HUAQING ZHU

Melatonin (MLT) is an endogenous indole compound with numerous biological activities that has been associated with atherosclerosis (AS). In the present study, rabbits were used as an AS model in order to investigate whether MLT affects endothelial cell permeability, myosin light chain kinase (MLCK) activity and MLCK expression via the mitogen-activated protein kinase (MAPK) pathway. Expression ...

Journal: :Diabetes 2000
W Yu T Niwa T Fukasawa H Hidaka T Senda Y Sasaki I Niki

Protein phosphorylation by myosin light-chain kinase (MLCK), protein kinase A, and protein kinase C (PKC) plays a positive role in insulin secretion from the pancreatic beta-cell. To investigate the underlying mechanisms, we examined intracellular distribution of the insulin granules and MLCK by immunofluorescence and immunoelectron microscopies and also investigated intracellular traffic of th...

Journal: :The Journal of Cell Biology 1991
D J Fishkind L G Cao Y L Wang

Myosin light chain kinase (MLCK) is thought to regulate the contractile activity in smooth and non-muscle cells, and may play an important role in controlling the reorganization of the actin-myosin cytoskeleton during cell division. To test this hypothesis we have microinjected the 61-kD catalytic fragment of MLCK into mitotic cells, and examined the effects of unregulated MLCK activity on cell...

Journal: :American journal of physiology. Cell physiology 2002
Emily K Blue Zoe M Goeckeler Yijun Jin Ling Hou Shelley A Dixon B Paul Herring Robert B Wysolmerski Patricia J Gallagher

To better understand the distinct functional roles of the 220- and 130-kDa forms of myosin light chain kinase (MLCK), expression and intracellular localization were determined during development and in adult mouse tissues. Northern blot, Western blot, and histochemical studies show that the 220-kDa MLCK is widely expressed during development as well as in several adult smooth muscle and nonmusc...

Journal: :American journal of physiology. Lung cellular and molecular physiology 2007
Jian Wang Letitia Weigand Joshua Foxson Larissa A Shimoda J T Sylvester

Antagonists of myosin light chain (MLC) kinase (MLCK) and Rho kinase (ROK) are thought to inhibit hypoxic pulmonary vasoconstriction (HPV) by decreasing the concentration of phosphorylated MLC at any intracellular Ca(2+) concentration ([Ca(2+)](i)) in pulmonary arterial smooth muscle cells (PASMC); however, these antagonists can also decrease [Ca(2+)](i). To determine whether MLCK and ROK antag...

2013
Hui-Jun Yu Leonid A. Serebryannyy Madeline Fry Madelyne Greene Olga Chernaya Wen-Yang Hu Teng-Leong Chew Nadim Mahmud Shrihari S. Kadkol Sarah Glover Gail Prins Zuzana Strakova Primal de Lanerolle

Many tumors are stiffer than their surrounding tissue. This increase in stiffness has been attributed, in part, to a Rho-dependent elevation of myosin II light chain phosphorylation. To characterize this mechanism further, we studied myosin light chain kinase (MLCK), the main enzyme that phosphorylates myosin II light chains. We anticipated that increases in MLCK expression and activity would c...

Journal: :The American journal of physiology 1997
Yuji Iida Takao Senda Yoshihisa Matsukawa Koji Onoda Jun-Ichi Miyazaki Hiromi Sakaguchi Yuji Nimura Hiroyoshi Hidaka Ichiro Niki

The aim of this study was to investigate how insulin secretion is controlled by phosphorylation of the myosin light chain (MLC). Ca2+-evoked insulin release from pancreatic islets permeabilized with streptolysin O was inhibited by different monoclonal antibodies against myosin light-chain kinase (MLCK) to an extent parallel to their inhibition of purified MLCK. Anti-MLCK antibody also inhibited...

Journal: :Journal of immunology 2005
Rafael L Simões Iolanda M Fierro

Lipoxins (LX) are arachidonic acid metabolites able to induce monocyte chemotaxis in vitro and in vivo. Nonetheless, the signaling pathways mediating this process are yet unclear. In this study, we have investigated the mechanisms associated with human monocyte activation in response to 15-epi-16-(para-fluoro)-phenoxy-LXA4 (ATL-1), a stable 15-epi-LXA4 analog. Our results demonstrate that ATL-1...

Journal: :American journal of physiology. Cell physiology 2006
Min Tae Kim Byung Joo Kim Jae Hwa Lee Seong Chun Kwon Dong Soo Yeon Dong Ki Yang Insuk So Ki Whan Kim

The classic type of transient receptor potential channel (TRPC) is a molecular candidate for Ca(2+)-permeable cation channels in mammalian cells. Because TRPC channels have calmodulin (CaM) binding sites at their COOH termini, we investigated the effect of CaM on mTRPC5. TRPC5 was initially activated by muscarinic stimulation with 50 microM carbachol and then decayed rapidly even in the presenc...

Journal: :The Biochemical journal 1997
A Sobieszek O Y Andruchov K Nieznanski

Telokin is an abundant smooth-muscle protein with an amino acid sequence identical with that of the C-terminal region of smooth-muscle myosin light-chain kinase (MLCK), although it is expressed as a separate protein [Gallagher and Herring (1991) J. Biol. Chem. 266, 23945-23952]. Here we demonstrate that telokin is also similar to smooth-muscle myosin regulatory light chain (ReLC) not only in it...

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