Mitotic activity is one of the most reliable prognostic factors in meningiomas. The identification of mitotic figures (MFs) and the areas of highest mitotic activity in H&E-stained slides is a tedious and subjective task. Therefore, we compared the results from immunostaining for the mitosis-specific antibody anti-phosphohistone H3 (PHH3 mitotic index [MI]) with standard MF counts (H&E MI) and ...

Correct orientation of the mitotic spindle in stem cells underlies organogenesis. Spindle abnormalities correlate with cancer progression in germ line-derived tumors. We discover a macromolecular complex between the scaffolding protein Gravin/AKAP12 and the mitotic kinases, Aurora A and Plk1, that is down regulated in human seminoma. Depletion of Gravin correlates with an increased mitotic inde...

BACKGROUND We evaluated the relation of proliferative indices with clinicopathological features and prognosis in breast cancer (BC) of Libyan female patients. The data were compared with corresponding results in Finland and Nigeria. PATIENTS AND METHODS Histological samples of breast cancer from 130 patients were retrospectively studied. Mitotic activity index (MAI) and standardized mitotic i...

BACKGROUND Prolonged mitotic arrest in response to anti-cancer chemotherapeutics, such as DNA-damaging agents, induces apoptosis, mitotic catastrophe, and senescence. Disruptions in mitotic checkpoints contribute resistance to DNA-damaging agents in cancer. MAD2 has been associated with checkpoint failure and chemotherapy response. In this study, a novel splice variant of MAD2, designated MAD2γ...

In order to develop a method for obtaining mitotic synchrony in aspergillus nidulans, we have characterized previously isolated heat-sensitive nim mutations that block the nuclear division cycle in interphase at restrictive temperature. After 3.5 h at restrictive temperature the mitotic index of a strain carrying one of these mutations, nimA5, was 0, but when this strain was subsequently shifte...

We describe a new mitotic locus of Drosophila melanogaster required for the progression through mitosis in the syncytial embryo and in late larval development. The locus aar (abnormal anaphase resolution) maps to the cytological interval 85E7-F16 and was identified by two alleles. The aar1 allele causes pupal lethality. Larval neuroblasts show an elevated mitotic index with high chromosome cond...

The influence of γrays on genetic variability is in direct correlation with the condition of irradiated material. Here we tried to increase the variability of Vicia faba L. var. major karyotype, after contamination with I (a kind of radioactive rain) in elongation phase in ten plant groups. The effects of irradiation were observed not only by controlling mitotic activity and calculating mitotic...

Medicinal plants are an inexhaustible source of molecules with several biological activities. The main objective this research was the evaluation antimitotic and genotoxic effect by Allium cepa test methanolic extract leaves Peganum harmala, a spontaneous plant from arid zones Algeria (Timzerth, Laghouat). Different concentrations (1, 4, 10, 16 mg∙mL-1) extract, as well two standards (colchicin...

The increased use of biostimulants in conventional agriculture and organic farming requires the implementation rapid tests to determine their effectiveness enhancing plant growth protection against abiotic stresses. However, innocuity health has rarely been demonstrated. We used Vicia faba Micronucleus Assay, as described by standard AFNOR EN ISO 29200(2020-05) reveal biostimulant, genotoxic cy...

The genotoxic potential of blitox (fungicide) was investigated by using chromosome aberration in Allium cepa root tip cells. Allium cepa roots were treated with 2g/lt., 3g/lt. and 4g/lt. concentrations of blitox and distilled water as control at 4 hours, 8 hours and 12 hours duration. The results indicated that blitox significantly increased the genetical abnormalities at all concentrations and...