Osteosarcoma (OS) is the most common primary malignant bone tumor in children, and microRNA-34a (miR-34a) replacement therapy represents a new treatment strategy. This study was to define the effectiveness and safety profiles of a novel bioengineered miR-34a prodrug in orthotopic OS xenograft tumor mouse model. Highly purified pre-miR-34a prodrug significantly inhibited the proliferation of hum...

Objective: MicroRNA-34a (miR-34a) is a tumor suppressor gene, which inhibits abnormal cell growth by regulating the expression of other genes. However, the role of miR-34a regulation sirtuin 1 (SIRT1) in prostate cancer is not clear. The objective of this study was to investigate the biological function and molecular mechanisms of miR-34a regulation SIRT1 in human prostate cancer PC-3 cell. Met...

MicroRNAs (miRNAs) are small non-coding RNAs composed of 18-25 nucleotides that regulate the expression of approximately 30% of human protein coding genes. Dysregulation of miRNAs plays a pivotal role in the initiation and progression of malignancies. Our study has shown that microRNA-34a (miR-34a) was upregulated in human endometrial cancer stem cells (ECSCs). However, it is unknown how miR-34...

Development of anticancer treatments based on microRNA (miRNA/miR) such as miR-34a replacement therapy is limited to the use of synthetic RNAs with artificial modifications. Herein, we present a new approach to a high-yield and large-scale biosynthesis, in Escherichia coli using transfer RNA (tRNA) scaffold, of chimeric miR-34a agent, which may act as a prodrug for anticancer therapy. The recom...

The Kaposi's sarcoma-associated herpesvirus (KSHV)-encoded chemokine receptor vGPCR acts as an oncogene in Kaposi's sarcomagenesis. Until now, the molecular mechanisms by which the vGPCR contributes to tumor development remain incompletely understood. Here, we show that the KSHV-vGPCR contributes to tumor progression through microRNA (miR)-34a-mediated induction of genomic instability. Large-sc...

Breast cancer severely affects women’s health. The role of microRNA (miR) in the pathological process of tumors has been widely studied as one novel biological marker for diagnosis, typing and prognosis. Previous study has suggested the correlation between breast cancer and miR. This study thus investigated expression level of miR-155 and miR-34a in both blood and tissue samples from breast can...

Purpose:Here,wedeterminedwhether epigenetic inactivationofmiR-34a andmiR-34b/c genesmay serve as a prognostic marker for distant metastases in colon cancer. Experimental Design: Using a case–control study design of 94 primary colon cancer samples with and without liver metastases, we determined CpG methylation frequencies of miR-34a and miR-34b/c promoters, expression of miR-34a, and its target...

MicroRNA-34 (miR-34), in particular miR-34a, has a negative regulatory effect on osteosarcoma cell proliferation, migration and invasion. Notably, it is also a post‑transcriptional regulatory factor of (sex determining region Y)‑box 2 (Sox-2), which is required for osteosarcoma cell self‑renewal and tumorigenesis. As a direct regulator of Sox‑2, miR‑34a has been hypothesized to be greatly assoc...

MicroRNA-34a (miR-34a) is a tumor suppressor that has attracted considerable attention in recent years. It modulates cancer cell invasion, metastasis, and drug resistance, and has also been evaluated as a diagnostic and/or prognostic biomarker. A number of targets of miR-34a have been identified, including some other non-coding RNAs, and it is believed that the modulation of these myriads of ta...

The pathology of proliferative vitreoretinopathy and proliferative diabetic retinopathy is linked to proliferation, migration, and adhesion of the retinal pigment epithelium. MicroRNA-34a (miR-34a) expression modulates changes in proliferation and migration of retinal pigment epithelial cell line ARPE-19. In this study, we determined that miR-34a interacts with LGR4, identified by bioinformatic...