objective: the aim of the present study was the production of recombinant lentviruses that express mir-16. after transduction, altered expression levels of mirna and its target protein were analyzed. methods: a dna fragment that contained the mir-16 precursor was cloned in a lentiviral plasmid. lentiviral vector particles were produced by transient calcium phosphate co-transfection of 293t cell...

Epilepsy is a chronic clinical syndrome of brain function which is caused by abnormal discharge of neurons. MicroRNAs (MiRNAs) are small noncoding RNAs which act post transcriptionally to regulate negatively protein levels. They affect neuroinflammatory signaling, glial and neuronal structure and function, neurogenesis, cell death, and other processes linked to epileptogenesis. The aim of this ...

Objective(s):An earlier meta-analysis on gene expression data derived from four microarray, two cDNA library, and one SAGE experiment had identified RGS5 as one of only ten non-housekeeping genes that were reported to be expressed in human trabecular meshwork (TM) cells by all studies. RGS5 encodes regulator of G-protein signaling-5. The TM tissue is the route of aqueous fluid outflow, and is r...

conclusions our findings introduced a novel therapeutic effect of diet therapy on circulating mirnas in obese patients with t2dm. mir-33b is an important therapeutic target in the treatment and prevention of t2dm and its complications. results the circulating levels of mir-29a and mir-33b in the diabetic women were higher (0.40-fold) and lower (1.43-fold), respectively, than normal levels. diet...

Objective(s): microRNA-29 (miR-29) family miRNAs have been mentioned as tumor suppressive genes in several human cancers. The purpose of this study was to investigate the function of miR-29a in nasopharyngeal carcinoma (NPC) cells. Materials and Methods: Human NPC cell line 5-8F was transfected with mimic, inhibitor or scrambled controls...

Abstract Background and aim Malignant involvement of the pericardium is seen in 1 to 20 percent autopsies patients with cancer. The most common metastatic tumor involving lung cancer [1]. We aimed distinguish origin pericardial fluid effusion (i.e. pericarditis vs cancer) based on miRNAs expression peripheral blood plasma. Method 8 hospitalized for collection neoplastic aetiology (lung cancer)....

In this paper, 4 microRNAs and their targets were predicted through a de novo prediction procedure in the genome of Porcine Reproductive and Respiratory Syndrome virus. Through clustalW analysis, these 4 microRNAs were recognized as novel PRRSV derivate microRNAs. Target prediction results showed PRRSV mir-1, 2 and 4 have total 16 targets in porcine 3’ UTR database. These targets could be divid...

Dieser Beitrag präsentiert ein webbasiertes Serious Game, das Studierenden in medizinischen Studiengängen die Möglichkeit gibt, Arzt-PatientenGespräche in Form von Rollenspielen mit simulierten Patienten selbständig und systematisch zu trainieren. Der Phase der Immersion (Rollenspiel) folgt dabei eine Phase der distanzierten Reflexion, um den lernförderlichen Perspektivenwechsel zu unterstützen...