نتایج جستجو برای: minigenome

تعداد نتایج: 153  

Journal: :The Journal of general virology 2008
Cyril Masante Kathleen Mahias Sofia Lourenço Estelle Dumas Annie Cahour Pascale Trimoulet Hervé Fleury Thérèse Astier-Gin Michel Ventura

Computer analysis of 158 hepatitis C virus (HCV) 5' untranslated region (5' UTR) sequences from the six genotypes showed that the 5' UTR from genotype 3 displays seven specific non-contiguous nucleotide changes, at positions 8, 13, 14, 70, 97, 203 and 224. The purpose of this study was to investigate the impact of these changes on translation and replication activities. Indeed, these modificati...

2016
Luis Martínez-Sobrido Juan Carlos de la Torre

Several arenaviruses cause hemorrhagic fever (HF) disease in humans and pose an important public health problem in their endemic regions. To date, no Food and Drug Administration (FDA)-licensed vaccines are available to combat human arenavirus infections, and current anti-arenaviral drug therapy is limited to an off-label use of ribavirin that is only partially effective. The development of are...

Journal: :Journal of virology 2005
Ana B Sánchez Juan C de la Torre

The arenavirus L protein has the characteristic sequence motifs conserved among the RNA-dependent RNA polymerase L proteins of negative-strand (NS) RNA viruses. Studies based on the use of reverse-genetics approaches have provided direct experimental evidence of the key role played by the arenavirus L protein in viral-RNA synthesis. Sequence alignment shows six conserved domains among L protein...

Journal: :Journal of virology 2003
Daniel D Pinschewer Mar Perez Juan Carlos de la Torre

The prototypic arenavirus lymphocytic choriomeningitis virus (LCMV) has a bisegmented negative-strand RNA genome. Each segment carries two viral genes in opposite orientation and separated by an intergenic region (IGR). The RNA-dependent RNA polymerase (RdRp) L of LCMV produces subgenomic mRNA and full-length genomic and antigenomic RNA species in two different processes termed transcription an...

Journal: :Journal of virology 2002
Michael Weik Jens Modrof Hans-Dieter Klenk Stephan Becker Elke Mühlberger

The nucleocapsid protein VP30 of Ebola virus (EBOV), a member of the Filovirus family, is known to act as a transcription activator. By using a reconstituted minigenome system, the role of VP30 during transcription was investigated. We could show that VP30-mediated transcription activation is dependent on formation of a stem-loop structure at the first gene start site. Destruction of this secon...

2012
Emilio Ortiz-Riaño Benson Y. H. Cheng Juan C. de la Torre Luis Martínez-Sobrido

Arenaviruses merit significant interest because several family members are etiological agents of severe hemorrhagic fevers, representing a major burden to public health. Currently, there are no FDA-licensed vaccines against arenaviruses and the only available antiviral therapy is limited to the use of ribavirin that is partially effective. Arenavirus nucleoprotein (NP) is found associated with ...

Journal: :The Journal of general virology 2007
Clive J Naylor Roger Ling Nicole Edworthy Carol E Savage Andrew J Easton

A prototype avian metapneumovirus (AMPV) vaccine (P20) was previously shown to give variable outcomes in experimental trials. Following plaque purification, three of 12 viruses obtained from P20 failed to induce protection against virulent challenge, whilst the remainder retained their protective capacity. The genome sequences of two protective viruses were identical to the P20 consensus, where...

Journal: :Journal of virology 2013
Minako Ito Masaharu Iwasaki Makoto Takeda Takanori Nakamura Yusuke Yanagi Shinji Ohno

Most viruses possess strategies to circumvent host immune responses. The measles virus (MV) nonstructural C protein suppresses the interferon response, thereby allowing efficient viral growth, but its detailed mechanism has been unknown. We identified Shc Src homology 2 domain-binding protein 1 (SHCBP1) as one of the host proteins interacting with the C protein. Knockdown of SHCBP1 using a shor...

2014
Gregor D. Wallat Qinfeng Huang Wenjian Wang Haohao Dong Hinh Ly Yuying Liang Changjiang Dong

Lassa virus (LASV) causes deadly hemorrhagic fever disease for which there are no vaccines and limited treatments. LASV-encoded L polymerase is required for viral RNA replication and transcription. The functional domains of L-a large protein of 2218 amino acid residues-are largely undefined, except for the centrally located RNA-dependent RNA polymerase (RdRP) motif. Recent structural and functi...

Journal: :The Journal of general virology 2005
Ashley C Banyard Michael D Baron Thomas Barrett

Rinderpest virus (RPV) is a morbillivirus that causes cattle plague, a disease of large ruminants. The viral genome is flanked at the 3' and 5' genome termini by the genome promoter (GP) and antigenome promoter (AGP), respectively. These promoters play essential roles in directing replication and transcription as well as RNA encapsidation and packaging. It has previously been shown that individ...

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