Twelve accessions of tronchuda cabbage landraces were tested for their ability to produce embryos through microspore culture in NLN-13 medium. A sample from all the isolations was stained with DAPI for determination of microspore developmental stage. The relationship between the microspore developmental stage and the production of microspore-derived embryos was evaluated. Embryos were obtained ...

Abstract Triticale is becoming an increasingly important livestock crop production. This evidenced by increasing triticale-producing areas and improved yields. In addition, meeting the demand for cereals involves introduction of high-yielding stress-resistant varieties into breeding. vitro culture techniques can accelerate development new varieties. Therefore, it seems extremely to develop effi...

Successful anther/microspore culture largely depends on the use of microspores at appropriate developmental stages at the time of culture, which can be specific for each plant species and genotype. This study was carried out to determine the correlation between morphological characteristics, namely floral bud (FB) length and width and anther length, and specific microspore developmental stages ...

In the course of a study of pollen wall development in the Gramineae two interesting relationships were noted: the locule wall and tapetal cells were bordered by spheroids which were similar chemically (resistant to acetolysis) and morphologically to the pollen exine. The pollen exine as well as the wall of the spheroids was perforated by submicroscopic channels. In the mature anther the sphero...