We have identified a fast and easy method for finding microsatellite markers that utilizes genome screening with inter-simple sequence repeat (ISSR) primers to detect microsatellite regions and to obtain sequence information flanking one side of the microsatellites and a restriction-ligation technique with a specific adaptor to allow sequence walking to obtain sequence information flanking the ...

A microsatellite-enriched genomic library for marble goby (Oxyeleotris marmoratus), a freshwater fish of considerable economic value, was obtained. A total of 16 microsatellite markers were successfully isolated and characterized in a population from the Mekong River in Vietnam. These markers had 2-20 alleles with expected heterozygosity ranging from 0.0370 to 0.8927. Linkage equilibrium was ob...

Buddleja (butterfly bush) is a genus of common landscape plants in temperate and subtropical gardens. Substantial breeding has led to wealth diverse cultivars with varied pedigrees. Molecular markers would be useful tools for breeders others studying butterfly bush identify cultivars. We evaluated SSR developed fingerprint 11 determine whether they were cultivar identification. Markers Bud_03, ...

The identification of informative in silico polymorphic genomic and genic microsatellite markers by comparing the genome and transcriptome sequences of crop genotypes is a rapid, cost-effective and non-laborious approach for large-scale marker validation and genotyping applications, including construction of high-density genetic maps. We designed 1494 markers, including 1016 genomic and 478 tra...

autosomal dominant polycystic kidney disease (adpkd) is the most common genetic nephropathy, which is characterized by replacement of renal parenchyma with multiple cysts. in iran, the disease prevalence within the chronic hemodialysis patient population is approximately 8-10%. so far, three genetic loci have been identified to be responsible for adpkd. little information is available concernin...

UNLABELLED PREMISE OF THE STUDY We developed microsatellite markers for the endangered plant Primula boveana, the Sinai primrose, and assessed the cross-transferability of these markers to six related taxa. • METHODS AND RESULTS DNA sequences containing microsatellites were isolated from a microsatellite-enriched library. We obtained successful amplification of 13 microsatellite primer pai...

Analyses of genetic structure and gene flow would provide information on the distance, direction and rate of dispersal of genes in specific populations. Thirty-six microsatellite markers (MS) have been developed within specific regions containing genes controlling chicken body weight and quality. Based on genetic marker positions in the chicken genome, the approximate genetic distances of these...

Microsatellite loci are standard genetic markers for population genetic analysis, whereas single nucleotide polymorphisms (SNPs) are more recent tools that require assessment of neutrality and appropriate use in population genetics. Twelve SNP markers were used to describe the genetic structure of Diabrotica virgifera virgifera (LeConte; Coleoptera: Chrysomelidae) in the United States of Americ...