Prostaglandins are known to be involved in the regulation of local blood flow within human skeletal muscles during exercise, and the concentration of prostaglandins increases locally and systemically in response to exercise. The systemic release of prostaglandins can be inhibited by oral intake of nonsteroidal anti-inflammatory drugs (NSAIDs). However, to study the local role of prostaglandins,...

BACKGROUND Surgical trauma causes stress and inflammatory reactions with elevated serum free fatty acids (FFA) and glucose levels characteristic of intraoperative insulin resistance. Our aim was to compare microdialysis findings with those using the classical organ balance technique and to test the clinical feasibility of microdialysis during cardiac surgery. METHODS Nine diabetic and nine no...

BACKGROUND Microdialysis is a sampling technique based on controlling the mass transfer rate of different-sized molecules across a semipermeable membrane. Because the dialysis process has minimal effects on the surrounding fluid, it is viewed as a tool for continuous monitoring of human metabolites. In diabetes treatment, microdialysis probes have been used as sampling systems coupled to a gluc...

Abstract Glioblastoma (GBM) is the most prevalent and lethally recurrent central nervous system neoplasm, but its therapeutic advancement has stagnated for over 15 years. One major hurdle in front of clinical breakthroughs been relative lack real-time, treatment-responsive biomarkers that occur during therapy. The methods microdialysis microperfusion, now entering both pre-clinical settings, of...

OBJECTIVE The aim of this study was to monitor and compare metabolic changes in the skeletal muscle during coronary artery bypass grafting surgery with and without cardiopulmonary bypass (CPB) by means of interstitial microdialysis. Glucose, lactate, pyruvate and glycerol were assessed as markers of basic metabolism and tissue perfusion. METHODS Twenty patients undergoing surgical myocardial ...

We have previously shown that static muscle contraction induces the expression of c-Fos protein in neurons of the nucleus tractus solitarii (NTS) and that some of these cells were codistributed with neuronal NADPH-diaphorase [nitric oxide (NO) synthase]-positive fibers. In the present study, we sought to determine the role of NO in the NTS in mediating the cardiovascular responses elicited by s...

Changes in the microdialysis outflow-to-inflow (O/I) ratio for [(14)C]ethanol and (3)H(2)O were determined in the perfused rat hindlimb after increases and decreases in nutritive flow mediated by the vasoconstrictors norepinephrine (NE) and serotonin (5-HT), respectively. Microdialysis probes (containing 10 mM [(14)C]ethanol and (3)H(2)O pumped at 1 or 2 microl/min) were inserted through the ca...

Stallknecht, Bente, Joop Madsen, Henrik Galbo, and Jens Bülow. Evaluation of the microdialysis technique in the dog fat pad. Am. J. Physiol. 276 (Endocrinol. Metab. 39): E588–E595, 1999.—In the present study the microdialysis technique was evaluated in an isolated autoperfused dog fat pad. Concentrations of glucose, lactate, and glycerol were measured in interstitial fluid by microdialysis and ...