BACKGROUND The aim of this study was to examine the expressions of the bcl-2, bax, fas and c-myc apoptosis-related genes in benign prostatic hyperplasia (BPH) and prostate carcinoma (CaP) to determine whether significant differences exist within each disease and between the two groups of patients. The correlation between gene expression and tumour diameter, stage, Gleason score and serum PSA wa...

It has been suggested that leukemia is characterized by an impaired balance between the proliferation of blood cells and their capacity to undergo apoptosis. The aim of this study was to examine the expression of key molecules related to apoptosis (BCL-2, BAX, FAS, FAS-L) in children with acute lymphoblastic leukemia (ALL). Measurement of BCL-2 and BAX mRNA was performed by quantitative real-ti...

background: in this study, the protective effect of thymoquinone (tq) and nigella sativa seeds oil (nso) was evaluated against oxidative damages induced by ethanol in rats. methods: animals were treated with ethanol 40% daily by gavages once a day for 4 weeks. nso and tq were injected intraperitoneal once a day for 4 weeks. the histopathological examination of liver, kidney, brain and heart was...

conclusions in general, it seems that three-month exercise training was effective in reducing cardiac mitochondrial pro-apoptotic protein. however, considering the results of the bcl2 gene expression, more researches are needed to identify effects of exercise trainings on indices of myocardial apoptosis. objectives the aim of this study was to assess the effect of exercise training on bcl-2 and...

Depression is a complex disease characterized by a series of pathological changes. Research on depression is mainly focused on the changes in brain, but not on liver. Therefore, we initially explored the metabolic profiles of hepatic extracts from rats treated with chronic unpredictive mild stress (CUMS) by UPLC-Q-TOF/MS. Using multivariate statistical analysis, a total of 26 altered metabolite...

Background: Disruption in spermatogenesis and infertility occurred after local testicular heating. In this study in addition to evaluation the long term effects of experimental bilateral and unilateral cryptorchidism on sperm parameters and structure of mouse testis, the genes that involved in programmed cell death after cryptorchidism and treatment methods of decreasing the germ cells in semin...

Purpose: To determine the effect of melatonin in combination with capecitabine on proliferation and induction apoptosis MCF-7 SK-BR-3 breast cancer cell lines.Methods: The MTT assay (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide) was performed to investigate lines half-maximal concentration (IC50) index. Expression apoptotic markers, Bax, Bcl-2, caspase-3 were measured by polym...

Excessive use of Ketamine (KET) has a neurotoxic effect on the brain. This study explored Transient Receptor Potential Vanilloid 4 (TRPV4) KET-induced neurotoxicity in hippocampus. We extracted and identified rat hippocampal neuronal cells. The neurons were treated with different concentrations (0, 0.1, 1, 10, 100, 300 1000 μmol/L) KET (6, 12 24 hr). Cell viability was detected by cell counting...

Background: Both mitochondrial dysfunction and aerobic glycolysis are signs of growing aggressive cancer. If altered metabolism of cancer cell is intended, using the glycolysis inhibitor (2-deoxyglucose (2DG)) would be a viable therapeutic method. The AMP-activated protein kinase (AMPK), as a metabolic sensor, could be activated with metformin and it can also launch a p53-dependent metabolic ch...