Acetylcholine-induced currents were measured in dissociated muscle fibres from the mouse interosseus. Fast and slow desensitization could be distinguished, the latter ending in a steady-state current. The time constants of the two phases of current decrease and the steady-state current were decreased when the superfusing solution contained di- or trivalent cations in addition to the 0.2 mM Mg2+...

Cardiac troponin C (cTnC) is a key effector in cardiac muscle excitation-contraction coupling as the Ca2+ sensing subunit responsible for controlling contraction. In this study, we generated several FRET sensors for divalent cations based on cTnC flanked by a donor fluorescent protein (CFP) and an acceptor fluorescent protein (YFP). The sensors report Ca2+ and Mg2+ binding, and relay global str...

The subcellular distribution of neutral sphingomyelinase activity has been determined in rat liver. Neutral sphingomyelinase is present in the plasma membrane. This enzyme requires either Mg2+ or Mn2+ for full activity; these cations cannot be replaced by Co2+ or Ca2+. The plasma membrane sphingomyelinase is strongly inhibited by Hg2+. A small amount of neutral spingomyelinase activity appears ...

Bu çalışmada Davraz Dağı (Isparta) karbonat akiferindeki yeraltısuyunun hidrojeokimyasal evrimini belirlemek amacıyla, bölgenin tektonik ve jeolojik özelliklerine bağlı olarak suların kavramsal modeli tanımlanmıştır. İnceleme alanından toplam 21 adet temsili yeraltısuyu örneği alınmış üç farklı fasiyeste olduğu belirlenmiştir: (a) Ca-HCO3, (b) Ca-Mg-HCO3 (c) Ca-Mg-HCO3-SO4. Ölçülen kısmen yükse...

The Danjiang River basin is an important water source for the Middle Route of South-to-North Water Diversion Project. With shortage resources and increase in pollution pressure, it great significance to study hydrochemical characteristics surface risk human health posed by polluted bodies protection utilization resources. In this paper, 40 samples were collected analyzed innovatively adopting s...

The construction of new, artificially snowed, ski slopes and the accompanying infrastructure changes natural environment exerts pressure on ecosystems. This study examined impact operation a new slope, with its artificial snow production, quality nearby stream waters. research period covered time before, during after slope construction. Electrolytic conductivity (EC) pH were measured on-site, c...

Millimolar concentrations of monovalent cations enhance and divalent cations impede the redistribution (spill-over) of electronic excitation energy from Photosystem (PS) II to PS I in cation-depleted (sucrose-washed) thylakoids; this concept is based on chlorophyll a fluorescence and electron transport measurements over a narrow pH range around 7. We have tested the above concept in pea thylako...

We have begun to identify and characterize genes that are differentially expressed with low magnesium. One of these sequences conformed to the solute carrier SLC41A1. Real-time RT-PCR of RNA isolated from renal distal tubule epithelial [mouse distal convoluted tubule (MDCT)] cells cultured in low-magnesium media relative to normal media and in the kidney cortex of mice maintained on low-magnesi...

Fructose-1,6-bisphosphatase requires divalent cations (Mg2+, Mn2+, or Zn2+) for catalysis, but a diverse set of monovalent cations (K+, Tl+, Rb+, or NH(4)(+)) will further enhance enzyme activity. Here, the interaction of Tl+ with fructose-1,6-bisphosphatase is explored under conditions that support catalysis. On the basis of initial velocity kinetics, Tl+ enhances catalysis by 20% with a K(a) ...

In this study, highly ordered mesoporous silica material (SBA-15) functionalized with Diethyl 2 - (9 -fluorenyl) malonate as a flourophore is reported. The anchoring of flourophores to the hydroxyl group on SBA-15 surface was done with post synthesis method. The obtained materials were characterized by small and wide angle X-ray diffraction, N2 adsorption–desorption, Fourier transform infrared ...