One of the major issues with heterologous production of proteins in Pichia pastoris X33 under AOX1 promoter is repeated methanol induction. To obviate repeated methanol induction, methyl esters were used as a slow release source of methanol in lipase expressing mut+ recombinant. Experimental design was based on the strategy that in presence of lipase, methyl esters can be hydrolysed to release ...

Jatropha curcas L. is one of the seed plants that have lipase activity and can work well in hydrolysis reactions chemical synthesis. This study aims to determine reacts with castor oil organic solvents. In this research, solvents, especially hydrophobic were used reaction increase lipase's catalytic activity. The solvent has a hydrophobicity level between 2 4, namely hexane. research stages con...