[3-14C]Acetoacetate, injected subcutaneously into rats, was rapidly incorporated into hepatic cholesterol and fatty acids. Injection of radiolabeled acetoacetate, acetate, or glucose resulted in the preferential incorporation of acetoacetate into cholesterol. The postmitochondrial supernatant of a rat liver homogenate has the capacity to synthesize radiolabeled sterols from [3-14C]acetoacetate,...

This is an automated method for the kinetic measurement of the combined concentrations of acetoacetate and beta-hydroxybutyrate in a single channel of the "Multistat III" centrifugal analyzer. Acetoacetate is first reduced with high concentrations of NADH by catalysis with 3-hydroxybutyrate dehydrogenase (EC 1.1.1.30). This reaction mixture is diluted with excess NAD+. The endogenous beta-hydro...

a simple and efficient method for the synthesis of quinolines and polycyclic quinolines using li+ modified nanoporous na+-montmorillonite is described. this new nanocatalyst proceeds via friedlander annulation under solvent-free conditions. it describes our observations about a trend of catalytic activity of lithium cation in nanoporous na+-montmorillonite. in this study, several types of 1,3-d...

The title compound, C(24)H(22)O(4)S, was prepared by reaction between (2E)-3-(6-meth-oxy-2-naphth-yl)-1-(2-thien-yl)prop-2-en-1-one and ethyl acetoacetate. In the crystal, the cyclo-hexenone ring shows a distorted half-chair conformation. The length of the double bond in the cyclohexenone ring [1.343 (4) Å] is normal.

The oxidation of fatty acids to acetoacetate or to carbon dioxide is affected by the presence of various aliphatic and aromatic acids. For example, benzoate inhibits acetoacetate formation from butyrate at low concentrations in guinea pig liver slices (Jowett & Quastel, 1935b) and in 'cyclophorase' preparations (Grafflin & Green, 1948). Benzoate also inhibits the oxidation of butyrate to C02 in...

Experiments with labeled octanoic acid have shown that liver slices cleave the fatty acid to 2-carbon fragments which condense to form acetoacetate (l-3). The earlier findings (1, 2) of an equal distribution of the isotope between the carboxyl and carbonyl groups of acetoacetate after utilization of carboxyl-labeled octanoate indicate a random recondensation of 2-carbon fragments, while later f...

1. Utilization of 5mM-glucose by slices of lactating mammary gland was decreased 33% on addition of acetoacetate (2mM) to the incubation medium. This inhibition was accompanied by increases in the intracellular concentrations of citrate and glucose 6-phosphate. 2. In the presence of acetoacetates the accumulation of pyruvate in the medium approximately doubled. 3. Insulin completely reversed th...

Several authors have recently re-emphasized the positive interference of acetoacetate in the measurement of creatinine using the Jaff6 reaction [1-3]. On theoretical grounds, the measurement of creatinine in the presence of acetoacetate should be possible using a kinetic method [4]. In practice, however, measurements by some instruments employing a kinetic Jaff6 method suffer from marked interf...

In Paper V of this series (1) it was found that the citrate which accumulates during the oxidation of W-tagged acetoacetate by rat kidney in the presence of oxalacetate had a high content of isotopic carbon, thus confirming the participation of tricarboxylic acids in the oxidation of fatty acid intermediates. The present study represents a continuation of the investigation of this reaction with...