Adding greenery from public spaces to the co-digestion process with sewage sludge was evaluated by shredding experiments and laboratory-scale batch and continuous mesophilic anaerobic fermentation experiments. The ratio of the shredded grass with 20mm or less in length by a commercially available shredder was 93%. The methane production was around 0.2NL/gVS-grass in the batch experiment. The co...

On a global scale agriculture and in particular enteric fermentation in ruminants is reported to produce about one fourth (21 to 25%) of the total anthropogenic emissions of methane (CH4). Methane is produced during the anaerobic fermentation of hydrolyzed dietary carbohydrates in the rumen and represents an energy loss to the host besides contributing to emissions of greenhouse gases into the ...

It has been suggested that the rumen microbiome and rumen function might be disrupted if methane production in the rumen is decreased. Furthermore concerns have been voiced that geography and management might influence the underlying microbial population and hence the response of the rumen to mitigation strategies. Here we report the effect of the dietary additives: linseed oil and nitrate on m...

Syngas biomethanation is a potent bio-conversion route, utilizing microorganisms to assimilate intermediate gases to produce methane. However, since methanogens have a long doubling time, the reactor works best at a low dilution rate; otherwise, the cells can be washed out during the continuous fermentation process. In this study, the performance of a practical reverse membrane bioreactor (RMBR...

Contribution of ruminants to total greenhouse gas emissions in Australia is approximately 10% and likely to increase with demand for livestock products, thus an efficient method of mitigation must be implemented. The red marine macroalgae Asparagopsis taxiformis reduces enteric methane production by up to 99% in vitro. Other macroalgae with less potent antimethanogenic properties may complement...

This study investigated the effects of disodium fumarate (DF) on methane emission, ruminal fermentation and microbial abundance in goats under different forage (F) : concentrate (C) ratios and fed according to maintenance requirements. Four ruminally fistulated, castrated male goats were used in a 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments and the main factors be...

Ruminants obtain nutrients from microbial fermentation of plant material, primarily in their rumen, a multilayered forestomach. How the different layers of the rumen wall respond to diet and influence microbial fermentation, and how these process are regulated, is not well understood. Gene expression correlation networks were constructed from full thickness rumen wall transcriptomes of 24 sheep...

Our study was undertaken in order to determine the effects of preliminary hydrothermal depolymerization and enzymatic hydrolysis of macroalgae biomass originating from the Vistula Lagoon on yield of the methane fermentation process in terms of quantity and quality of produced biogas. The process of enzymatic hydrolysis was conducted with a mixture of enzymes: Cellulast 1.5 L, Novozym 188, and H...

Anaerobic bacteria from a Pacific Ocean mud cause unusually large carbon isotope fractionations during fermentation of methanol. The methane produced is about 8 percent enriched in C(12), relative to the original methanol. Fractionation factors determined at 30 degrees C and 23 degrees C were 1.081 and 1.094, respectively, which indicates that this process is dependent on temperature.

The objective of this in vitro study was to compare fermentation patterns of seven selected traditional medicinal plants and investigate the effects of medicinal plant mixture (MPM) supplements and a high-concentrate diet (lucerne hay and barley grain, LH+BG, 40:60) on rumen fermentation and fatty acid composition. An MPM of Taraxacum officinale L., Acorus calamus L., Calendula officinalis L., ...