n-CdZnS thin films have been prepared by our in-house designed and developed chemical bath deposition (CBD) instrument using 2-mercaptoethanol as capping agent. The structural properties were investigated through X-ray diffraction (XRD) analysis which results the obtained CdZnS are of hexagonal phase. surface morphology texture fabricated characterized scanning electron microscopy atomic force ...

physicochemical properties. Deleya pacifica (Baumann et al. 1972) Baumann et al. 1983 IAM 141 15*1 was aerobically grown for 24hr at 30°C in a modified marine broth as described previously.2) The cells (30g) harvested from 6liters of culture broth by centrifugation were suspended in 200ml of a sonication buffer (pH 7.5) containing 10mM K2HPO4, 7mM 2-mercaptoethanol, 100mM NaCl and 20/xg/ml phen...

The extrusion apparatus of the microsporidian parasitic protozoan Nosema michaelis discharges an invasion (or polar) tube with a velocity suitalbe for piercing cells and injecting infective sporoplasm. The tube is composed of a polar tube protein (PTP) which consists of a single, low molecular weight polypeptide slightly smaller than chymotrypsinogen-A. Assembled PTP tubes resist dissociation i...

AIMS To verify the improved thiol methyltransferase (TMT) assay and measure activity in isolated colonocytes and erythrocyte membranes of the same subjects. METHODS High performance liquid chromatography with radioactivity detection was used to measure 14C-methylmercaptoethanol formation, the reaction product of cell extracts incubated with mercaptoethanol and 14C-S-adenosylmethionine. RESU...

2-Mercaptoethanol increases the optical density of assay solutions at wavelengths between 280 to 400 nm, and therefore interferes with the measurement of protein concentration by the microbiuret method. Protein concentration can be determined in the presence of 2-mercaptoethanol up to 6 mM by modification of the method as follows: after the precipitation of protein by trichloroacetic acid in th...

A kininogenin (EC 3.4.21.8) was purified from the venom of Vipera ammodytes ammodytes (European sand viper) by a combination of gel filtration and ion-exchange chromatography. The enzyme is approximately six times more active than bovine trypsin in its ability to release vasoactive peptides from a plasma precursor. The kininogenin is a glycoprotein containing 18-20% by weight of carbohydrate. I...

Rats thymectomized and irradiated as adults were restored to immunologic reactivity by grafts of normal adult rat thymus and bone marrow. Reactivity of the delayed (cellular) type and formation of mercaptoethanol-sensitive (MES) and mercaptoethanol-resistant (MER) antibody returned within 3 weeks, while Arthus reactivity remained subnormal till 9 weeks after irradiation and grafting. When the t...

The characteristics of the intact factor VIll/von Willebrand factor protein binding to human platelets was compared to 2-mercaptoethanol-treated factor VIll/von Willebrand factor protein and to fractions of plasma factor VIll/von Willebrand factor protein that elute after the void volume. These studies indicate that the factor VIlI/von Willebrand factor protein larger size oligomers bind prefer...