this study was carried out to investigate effects of copper sulphate (cuso4) additive to semen extenders on sperm parameters: progressive motility, viability, membrane integrity and dna damage, after semen dilution and cryopreservation. semen samples of 5 buffalo bulls of 3-5 years old were collected at 5 different occasions during the autumn 2011. a total number of 25 samples were used in each...

Oxidative stress during freezing-thawing reduces sperm motility, viability, membrane functions and antioxidant capacity. Thymus vulgaris has antioxidant properties due to phenolic compounds. The purpose of current study was to evaluate the effect of Thymus vulgaris extract as a natural antioxidant on post-thawed Moghani ram sperm quality. Semen samples were collected twice a week from four rams...

Background: A number of studies have demonstrated membrane lipid peroxidation (LPO) as one of the causes of defective sperm function in liquid semen preserved at 4◦C and cryopreserved semen. Some attempt has been made to preserve sperm parameters, particularly sperm motility, by adding some elements and Materials to the semen before freezing. Materials and Methods: To investigate effects of cop...

Salicylic acid (SA) is considered to be plant signal molecule that plays a key role in plant growth, development, and defense responses. The physiological mechanism of exogenous SA to affect the senescence of cut lisianthus flowers during vase life was investigated. Fresh cut lisianthus flowers were treated with distilled water (control), 0.5, 1 and 2 mM SA and then held at 25◦C up to 12 days. ...

The anti-senescence biogenic polyamines (PAs) putrescine (Put), spermidine (Spd) and spermine (Spm) are involved in plant growth processes and responses to stress. The physiological mechanism of exogenous Put to affect the senescence of cut lisianthus flowers during vase life was investigated. Fresh cut lisianthus flowers were treated with distilled water (control), 0.5, 1 and 2 mM Put and then...

Ejaculates from 7 stallions were split and simultaneously frozen in three different extenders, INRA 96 egg yolk glycerol, Ghent and the newly developed extender Caceres. After thawing, samples were evaluated for motility (CASA system) sperm membrane integrity and early membrane changes (YoPro-1/Eth staining), acrosome integrity (FICT-PNA), and mitochondrial membrane potential (JC-1) (flow cytom...