نتایج جستجو برای: mediated isothermal amplification lamp

تعداد نتایج: 480656  

2017
Muneaki Hashimoto Hirokazu Sakamoto Yusuke Ido Shouki Yatsushiro Kazuaki Kajimoto Masato Tanaka Masatoshi Kataoka

Highly sensitive and rapid diagnostic methods that should also be appropriate for Point-of-Care (POC) testing are required for Mass Screening and Treatment (MSAT) programs to be effective in blocking transmission of malaria, and eventually eradicating the disease. Loop-Mediated Isothermal Amplification (LAMP) is an accurate, sensitive, rapid, and easy-to-perform method for gene amplification. P...

2012
Anthony Ablordey Diana Ackon Amissah Isaac Frimpong Aboagye Ben Hatano Toshio Yamazaki Tetsutaro Sata Koichi Ishikawa Harutaka Katano

BACKGROUND Buruli ulcer (BU) caused by Mycobacterium ulcerans (M. ulcerans) has emerged as an important public health problem in several rural communities in sub-Saharan Africa. Early diagnosis and prompt treatment are important in preventing disfiguring complications associated with late stages of the disease progression. Presently there is no simple and rapid test that is appropriate for earl...

2017
Yuka OKUWA Michiko MIYAMATO-HAYASHI Takaichi TANAKA Yuji HAYAKAWA Yasuo INOSHIMA

Various techniques for screening and detection of bovine leukemia virus (BLV) were compared to ascertain a rapid and simple technique for routine examination. The performance of real-time PCR, nested PCR and loop-mediated isothermal amplification (LAMP) assays was compared using DNA extracted from whole blood instead of white blood cells (WBCs) of 23 cattle. Real-time PCR, LAMP and nested PCR d...

2015
Sanchita Bhadra Yu Sherry Jiang Mia R. Kumar Reed F. Johnson Lisa E. Hensley Andrew D. Ellington

The Middle East respiratory syndrome coronavirus (MERS-CoV), an emerging human coronavirus, causes severe acute respiratory illness with a 35% mortality rate. In light of the recent surge in reported infections we have developed asymmetric five-primer reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays for detection of MERS-CoV. Isothermal amplification assays will fac...

Journal: :Japanese journal of infectious diseases 2009
Bal Ram Adhikari Basu Dev Pandey Prakash Ghimire Bhawana Shrestha Manoj Khadka Tomoko Yoda Yasuhiko Suzuki

Most first-line anti-tuberculosis drugs have less in vitro activity against atypical mycobacteria. Loop-mediated isothermal amplification (LAMP) was used for the rapid diagnosis of mycobacterial species. The sensitivity of LAMP was 96.1% (49/51) in smear-positive and culture-positive sputum samples and 85.0% (17/20) in smear-negative and culture-positive samples. Of the 77 total LAMP-positive s...

2014
Yao-zhong Ding Jian-hua Zhou Li-na Ma Yan-ni Qi Gang Wei Jie Zhang Yong-guang Zhang

A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed to rapidly detect foot-and-mouth disease virus serotype C (FMDV C). By testing 10-fold serial dilutions of FMDV C samples, sensitivity of the FMDV C RT-LAMP was found to be 10 times higher than that of conventional reverse transcription- PCR (RT-PCR). No cross-reactivity with A, Asia 1, or O FMDV or swi...

Journal: :jundishapur journal of microbiology 0
weihua yang medical research & laboratory diagnostic center, jinan central hospital, shandong university, jinan, 250013, p.r.china cuiming cao medical research & laboratory diagnostic center, jinan central hospital, shandong university, jinan, 250013, p.r.china weichen wang medical research & laboratory diagnostic center, jinan central hospital, shandong university, jinan, 250013, p.r.china yunshan wang medical research & laboratory diagnostic center, jinan central hospital, shandong university, jinan, 250013, p.r.china; medical research & laboratory diagnostic center, jinan central hospital, shandong university, jinan, 250013, p.r.china. tel: +86-53185695350

methods genomic dna of cultured s. agalactiae was prepared and loop-mediated isothermal amplification (lamp) primers were designed based on the camp gene in bacteria. the optimum primer set was selected based on the reaction speed and specificity. the reaction result was monitored visually. the sensitivity and specificity of the lamp method were evaluated and compared with polymerase chain reac...

2012
B. Wang S. H. Cai Y. S. Lu X. L. Zhang Y. C. Huang J. C. Jian Z. H. Wu

The purpose of this study was to develop a loop-mediated isothermal amplification (LAMP) method for rapid, sensitive, and simple detection of Streptococcus agalactiae in farmed fish. A set of four primers, two outer and two inner, was designed from the fibrinogen-binding (fbsB) proteins gene sequence of S. agalactiae, and conditions for LAMP were optimized as incubation of all reagents for 60 m...

2015
Yuta KINOSHITA Hidekazu NIWA Yoshinari KATAYAMA Kazuhisa HARIU

Taylorella equigenitalis is a causative bacterium of contagious equine metritis (CEM), and Taylorella asinigenitalis is species belonging to genus Taylorella. The authors developed two loop-mediated isothermal amplification (LAMP) methods, Te-LAMP and Ta-LAMP, for detecting T. equigenitalis and T. asinigenitalis, respectively. Using experimentally spiked samples, Te-LAMP was as sensitive as a p...

Journal: :international journal of advanced biological and biomedical research 2013
maryam tarighati vajihe karbasizade sharareh moghim

herpetic keratitis which is resulted from herpes simplex virus type i, is considered the most common cause of corneal blindness that needs being diagnosed and treated in order to prevent irreversible eye effects. the best method to diagnose it is molecular method which has high sensitivity and exclusivity. the current study was done on 25 eye samples collected from patients referred to the feyz...

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