Myoglobin (Mb) catalyzes various two-electron oxidations; however, ferryl porphyrin cation radical equivalent to peroxidase compound I has not been identified yet. Distal histidine mutants of sperm whale Mb (His-64 --> Ala, Ser, and Leu) afford an apparent intermediate followed by the formation of a ferryl heme (Mb-II) in the reaction with m-chloroperbenzoic acid. Because the intermediate exhib...

Myoglobin (Mb) interaction with the outer mitochondrial membrane (OMM) promotes oxygen (O2) release. However, comprehensive molecular details on specific contact regions of OMM oxygenated (oxy-) and deoxygenated (deoxy-)Mb are missing. We used dynamics (MD) simulations to explore oxy- deoxy-Mb lipids in two lipid compositions: (a) a typical whole average, (b) specifically cardiolipin-enriched c...

ndrew G. C. Sutton, MA, MB, MRCP,* Philip G. Campbell, MB, MRCP,* ichard Graham, MB, MRCP,* Dallas J. A. Price, MB, MRCP,* Janine C. Gray, BSC(HONS), PHD,† ver D. Grech, MD, MRCP, FACC,* James A. Hall, MA, MD, FRCP,* lun A. Harcombe, MD, MRCP,* Robert A. Wright, MD, FRCP,* oger H. Smith, BSC(HONS), MB, FRCP,‡ Jerry J. Murphy, MB, BS, DM, FRCP,§ nanthaiah Shyam-Sundar, MB, BS, MD, DM, FRCP,‡ Mic...

The present study aimed to investigate the roles of the vascular endothelial cell growth factor (VEGF) and micro (mi)RNA-210 in the metastasis of primary medulloblastoma (MB) tumors. A total of 86 adult patients diagnosed with cerebellar MB were enrolled in the present study, of which 11 patients had metastatic MB in the subarachnoid space. The following samples were collected: MB primary tumor...

Incidence, Event Rates, and Early Outcome of Stroke in Dublin, Ireland: The North Dublin Population Stroke Study Peter J. Kelly, MD; Gloria Crispino, PhD; Orla Sheehan, MB; Lisa Kelly, MB; Michael Marnane, MB; Aine Merwick, MB; Niamh Hannon, MB; Danielle Ní Chróinín, MB; Elizabeth Callaly, MB; Dawn Harris, BSc; Gillian Horgan, BSc; Emma B. Williams, PhD; Joseph Duggan, MD; Lorraine Kyne, MD; Pa...

Background:Organophosphates are commercially available agrochemicals for pest control, but may be abused for deliberate self-poisoning. We report a case of suicidal ingestion of dimethoate, a moderately toxic organophosphate insecticide, which can cause refractory hemodynamic instability, and the successful use of methylene blue (MB) for counteracting this complication. Case Presentation: An u...

Although drug resistance is often observed in metastatic recurrence of breast cancer, little is known about the intrinsic drug resistance in such metastases. In the present study, we found, for the first time, that MDA-MB-231BR, a brain metastatic variant of a human breast cancer cell line, was refractory to treatment with 5-fluorouracil (5-FU) even without chronic drug exposure, compared to it...