An easy, rapid and robust dipstick assay for detection of leptospira-specific immunoglobulin M (IgM) antibodies was evaluated on 403 patients admitted for hospitalization because of fever. The clinical symptoms and signs of 35 patients were consistent with leptospirosis. The final diagnosis for the remaining patients was as follows: 136 with typhoid fever, 82 with hepatitis, 74 with malaria, 48...

BACKGROUND Timely diagnosis of leptospirosis is essential for early and effective treatment, for there are many differential diagnoses for it.. Leptospiral researchers have an increasing interest in developing new serological methods with recombinant antigens to improve the Leptospirosis diagnosis. Several serological tests have been developed for the proper diagnosis of leptospirosis. OBJECT...

BACKGROUND Leptospirosis is a re-emerging infectious disease that is under-recognized due to low-sensitivity and cumbersome serological tests. MAT is the gold standard test and it is the only serogroup specific test used till date. Rapid reliable alternative serogroup specific tests are needed for surveillance studies to identify locally circulating serogroups in the study area. METHODS/PRINC...

BACKGROUND Diagnosis of leptospirosis by the microscopic agglutination test (MAT) or by culture is confined to specialized laboratories. Although ELISA techniques are more common, they still require laboratory facilities. Rapid Diagnostic Tests (RDTs) can be used for easy point-of-care diagnosis. This study aims to evaluate the diagnostic performance of the RDTs LeptoTek Dri Dot, LeptoTek Later...

Uveitis is considered a rare complication of leptospirosis. This report describes an epidemic of uveitis among patients with leptospirosis and provides data, using polymerase chain reaction (PCR) amplification of Leptospira DNA, that the pathogenesis is associated with anterior chamber spirochetes. Forty-six uveitis patients, 49 uveitis controls, and 54 cataract controls were enrolled at Aravin...

The present study compared the efficacy of Microscopic Agglutination Test (MAT) with UpL32 and UpL41 recombinant antigen based Enzyme-linked immunosorbent assay (ELISA) for the diagnosis of bovine leptospirosis. The UpL32 and 41 genes were amplified using specific primers and the polymerase chain reaction products (peR) were cloned. The gene inserts from positive recombinant clones were inserte...

BACKGROUND Uveitis is increasingly being reported from south India following epidemics of leptospirosis. The incidence of eye involvement in treated patients has not been investigated properly in prospective studies. AIMS To determine the incidence, clinical spectrum and risk factors for Leptospiral uveitis in antibiotic-treated patients. SETTINGS AND DESIGN A prospective cohort study condu...

We have developed a one-point microcapsule agglutination test (MCAT) for the serodiagnosis of leptospirosis. The MCAT kit was evaluated for use in humans by six WHO Collaborating Centres for Reference and Research on Leptospirosis. The laboratories classified their serum samples on the basis of the microscopic agglutination test (MAT) and the following screening tests: enzyme-linked immunosorbe...

Background: Slaughterhouse workers have a risk of contracting leptospirosis because daily contact with animal blood and urine. The purpose the study was to describe incidence measure level knowledge examine relationship between demographic characteristics, prevention behavior leptospirosis.Methods: All technical staff at Salatiga (30 people) were selected as participants. Data collected in July...

leptospirosis is an important infectious disease of animals and humans caused by the pathogenicleptospires which are classified into one species of leptospira interrogans containing over 212 serovars. thisstudy was conducted to determine the prevalence of leptospira-induced abortions in tabriz (north-west ofiran) dairy herds and to determine the pathogenic leptospira serovars responsible. from ...