When a derivatized oligosaccharide isolated from ovalbumin and containing 6 mannose residues was incubated with yeast membranes and GDP-mannose, two sets of products were obtained, a high molecular weight one containing about 25 mannose residues and a low molecular weight one consisting of compounds with 7, 8, and 9 mannose residues, respectively. When the low molecular weight products were rei...

results in this study, 45 (13.4 %) urine samples were positive for candida species (17 males and 28 females). the most common isolated yeast was candida albicans (34%), followed by c. glabrata (32.1%), c. tropicalis (9.4%), other candida species (22.6%), and rhodotorula species (1.9%). the mean serum levels of mbl were 0.85 ± 0.01 ng/ml and 1.02 ± 0.03 ng/ml among candiduric patients and contro...

When pig liver microsomal preparations were incubated with GDP-[(14)C]mannose, 10-40% of the (14)C was transferred to mannolipid and 1-3% to mannoprotein. The transfer to mannolipid was readily reversible and GDP was one of the products of the reaction. It was possible to reverse the reaction by adding excess of GDP and to show the incorporation of [(14)C]GDP into GDP-mannose. When excess of un...

Four wheat germ agglutiniti (WGA)-resistant variants of the highly metastatic murine tumor MDAY-D2 were found to have altered tumorigenic and metastatic capacities in the syngeneic DBA/2 host. The parental tumor metastasized to lungs (100%) and completely replaced the liver (100%) in the syngeneic DBA/2 host. In contrast, the variants formed large, discrete tumor nodules in the liver (100%) and...

Class IIa bacteriocins may be used as natural food preservatives, yet resistance development in the target organisms is still poorly understood. In this study, the understanding of class IIa resistance development in Listeria monocytogenes is extended, linking the seemingly diverging results previously reported. Eight resistant mutants having a high resistance level (at least a 10(3)-fold incre...

The cell surface glycopeptides from an epithelial cell line (CCL 239) derived from normal human colon were compared with those from three cell lines (HCT-8R, HCT-15, and CaCo-2) derived independently from human colonie adenocarcinomas. Cells were incubated with o-[2-3H]mannose or L-[5,6-3H]fucose for 24 h and treated with trypsin to release cell surface compo nents which were then digested exha...