Journal:
:medical journal of islamic republic of iran
0
fereshteh shahcheraghi from the department of microbiology, medical school, tarbiat modaress university, tehran,
mohammad bagher eslami from the department of microbiology, medical school, tarbiat modaress university, tehran,
bahman tabaraie the department of bacterial vaccine and antigen production, pasteur institute of iran, tehran, islamic republic of iran.
ghorban behzadian-nejad from the department of microbiology, medical school, tarbiat modaress university, tehran,
isolation and purification of major outer membrane proteins (omp) from the cell wall envelope of brucella abortus s-99 were achieved by sonication, solubilization and membrane fractionation in the presence of non-ionic detergent (tx-100) and lysozyme treatments, followed by ultracentrifugation. the crude omp was treated with trypsin to free the preparation from any other protein contaminants. t...
