Simple repeat polymorphisms, like (GT)n repeats are often multiallelic systems with high heterozygosity, which may substantially increase the number of informative families in linkage studies. Here I describe a rapid method for the isolation of (GT)n repeats from yeast artificial chromosomes (YACs), derived from approaches already described for the isolation of YAC ends (1), and for the isolati...

in order to identify the sex marker in mahisefied, rutilus frisii kutum, samples from 5 male and 5 female fish were collected from the south caspian sea. polymerase chain reaction random amplified polymorphic dna (pcr-rapd) was performed using 124 primer sets. all bands were numbered using 1 and 0 scores corresponding to the presence or absence of bands, respectively and data were analyzed usin...

In order to identify the sex marker in Mahisefied, Rutilus frisii kutum, samples from 5 male and 5 female fish were collected from the south Caspian Sea. Polymerase chain reaction random amplified polymorphic DNA (PCR-RAPD) was performed using 124 primer sets. All bands were numbered using 1 and 0 scores corresponding to the presence or absence of bands, respectively and data were analyzed usin...

Genetic diversity of six populations of bartail flathead (Platycephalus indicus Linnaeus, 1758) was investigated using amplified fragment length polymorphism (AFLP). A total of 118 reproducible bands amplified with ten AFLP primer combinations were obtained from 42 fishes that were collected from six different locations in the northern part of the Persian Gulf. The percentage of polymorphic ban...

Genetic diversity of six populations of bartail flathead (Platycephalus indicus Linnaeus, 1758) was investigated using amplified fragment length polymorphism (AFLP). A total of 118 reproducible bands amplified with ten AFLP primer combinations were obtained from 42 fishes that were collected from six different locations in the northern part of the Persian Gulf. The percentage of polymorphic ban...

BACKGROUND Chronic exposure of mammalian organism to morphine results in adaption to persistent high opioid tone through homeostatic adjustments. Our previous results indicated that in the frontal brain cortex (FBC) of rats exposed to morphine for 10 days, such a compensatory adjustment was detected as large up-regulation of adenylylcyclases I (8-fold) and II (2.5-fold). The other isoforms of A...

west part of iran is considered as one of the center of diversity for aegilops triunsialis, therefore, study of its genetic diversity in this area is extremely important regarding evolution of this plant. the genetic variation among 40 accessions of ae.triunsialis collected from west of iran were studied using 12 issr markers. dna was extracted, concentrations adjusted and five samples from eac...

Random amplified polymorphic DNA markers were used to evaluate the genetic diversity in a representative population of Terminalia pallida from six different geographical regions of chittoor district. 20 OPA primers were used for the RAPD analysis. A total of 33 bands yielded from 7 reproducible primers, with a mean of 4.28 amplified bands per primer. The plant species showed 69.5% polymorphism....

Sequence-related amplified polymorphism (SRAP) markers were used to analyze and estimate the genetic variability, level of diversity, and relationships among 20 cultivars and strains of annual ryegrass (Lolium multiflorum Lam.). Eighteen SRAP primer combinations generated 334 amplification bands, of which 298 were polymorphic. The polymorphism information content ranged from 0.4715 (me10 + em1)...

Random amplified polymorphic DNA (RAPD) bands that distinguish Meloidogyne hapla and M. chitwoodi from each other, and from other root-knot nematode species, were identified using a series of random octamer primers. The species-specific amplified DNA fragments were cloned and sequenced, and then the sequences were used to design 20-mer primer pairs that specifically amplified a DNA fragment fro...