Borrelia burgdorferi-related isolates were tested for their sensitivity to normal human serum (NHS) and their ability to activate complement. By dark-field microscopy, electron microscopy, and subsurface plating, it was shown that exposure of a Borrelia garinii isolate to 10% or more NHS resulted in immobilization, blebbing, and killing of the spirochetes. These effects were mediated by complem...

Staphylococcus epidermidis is a common pathogen in medical device-associated infections. Its major pathogenetic factor is the ability to form adherent biofilms. The polysaccharide intercellular adhesin (PIA), which is synthesized by the products of the icaADBC gene cluster, is essential for biofilm accumulation. In the present study, we characterized the gene locus inactivated by Tn917 insertio...

The number of edges in a shortest walk (without repetition vertices) from one vertex to another connected graph G is known as the distance between them. For id="M2"> x and an edge id="M3"> e = a b id="M4"> , minimum distances id="M5"> with id="M6"> id="M7"> said be id="M8"> id...

The organotin(IV) compounds, [Ph3SnL H]n Æ nCCl4 (1), [Me2SnL (OH2)] (2), [ Bu2SnL ] (3), [Ph2SnL ]n (4), [Ph3SnL H]n (5) and [Ph3SnL H]n (7) (L 1 = 2-{[(2Z)-(3-hydroxy-1-methyl-2-butenylidene)]amino}phenylpropionate and L 3 = 2-{[(E)-1-(2-hydroxyaryl)alkylidene]amino}phenylpropionate), were synthesized by treating the appropriate organotin(IV) chloride(s) with the potassium salt of the ligand ...

To explore the metabolism of 1,5-dicaffeoylquinic acid (1,5-DCQA) in rats, liquid chromatography-mass spectrometry in parallel to diode-array detection was used for the rapid detection/characterization of the metabolites formed in bile, urine, and plasma of rats following oral administration of 1,5-DCQA (160 mg/kg). The methylation and glucuronidation of 1,5-DCQA occurring in vitro using rat li...

Osteoarthritis is a common subtype of musculoskeletal disorder (MSD) among older adults. It associated with increased swelling, inflammation, decreased range motion, and joint instability significant implications on health-related quality life (HRQoL). This study compared HRQoL patients osteoarthritis other types MSDs. The nationally representative sample was selected from the 2011-2019 Medical...

GSH metabolism in yeast is carried out by the gamma-glutamyl cycle as well as by the DUG complex. One of the last steps in the gamma-glutamyl cycle is the cleavage of Cys-Gly by a peptidase to the constitutent amino acids. Saccharomyces cerevisiae extracts carry Cys-Gly dipeptidase activity, but the corresponding gene has not yet been identified. We describe the isolation and characterization o...

Hepatocyte growth factor activator inhibitor-1 (HAI-1) was initially identified as cognate inhibitor of matriptase, a membrane-bound serine protease. Paradoxically, HAI-1 is also required for matriptase activation, a process that requires sphingosine 1-phosphate (S1P)-mediated translocation of the protease to cell-cell junctions in human mammary epithelial cells. In the present study, we furthe...

Induced mutagenesis causes an increase variation of some products with limited genetic resources. In this study, the antioxidant enzymes activity, biochemical properties and glycoside content of Stevia were assessed by EMS (chemical mutagen) based on two experiments as factorial with completely randomized design with three replications. The results of the first experiment showed that some prope...

Background Activation of Type I interferons (IFNs) and a wide array innate adaptive immune mediators are hallmarks the pathogenesis systemic lupus erythematosus (SLE) activation IFN pathways correlates with disease activity [1]. In phase 2 study SLE patients, upadacitinib (UPA, Janus kinase inhibitor) given alone or in combination elsubrutinib (ABBV-599, Bruton’s tyrosine resulted significant i...