BACKGROUND & OBJECTIVES Mycoplasma pneumoniae is the most important and common cause of community-acquired pneumonia (CAP). The conventional detection methods (culture and serology) lack sensitivity. PCR offers a better approach for rapid detection but is prone to carry over contamination during manipulation of amplification products. Quantitative real-time PCR (qRT-PCR) method offers an attrac...

The protistan parasite Mikrocytos mackini, the causative agent of Denman Island disease in the oyster Crassostrea gigas in British Columbia, Canada, is of wide concern because it can infect other oyster species and because its life cycle, mode of transmission, and origins are unknown. PCR and fluorescent in situ hybridization (FISH) assays were developed for M. mackini, the PCR assay was valida...

Gibel carp Carassius auratus gibelio (Bloch), a commercially important freshwater-cultured fish in China, is threatened by myxosporeans, particularly Thelohanellus wuhanensis, Myxobolus honghuensis, M. wulii and M. turpisrotundus. Here, we developed a multiplex PCR assay for simultaneous detection of these 4 myxosporeans. The specific primers for each species were designed based on the 28S rDNA...

The diagnosis of pneumonia caused by Mycoplasma hyopneumoniae remains frustrating. To increase our ability to detect M. hyopneumoniae, we modified our present M. hyopneumoniae PCR assay to enable us to quantify the number of organisms in a sample and to allow the detection of the organism in formalin fixed and paraffin embedded lung tissue samples. An ELISA-based M. hyopneumoniae PCR assay util...

this study was conducted to determine presence and distribution of arcobacter spp. by conventional culture and a multiplex pcr (m-pcr) in the intestinal content samples that are collected from the cattle, sheep and goats and in the faecal samples of people who reffered to the hospitals in the east of turkey, because of complains about gastroenteritis and diarrhea. in the examination of the tota...

tuberculosis is a serious global public health problem and its high prevalence is stron gly associated with the enhancement of drug resistance. in this study we demonstrate a multiplex allele-specific polymerase chain reaction (mas)-pcr assay to simultaneously detect mutations in the first and third bases of the embb gene codon 306 atg in ethambutol (emb) resistant isolates of mycobacterium tub...

the genus campylobacter  is of great importance to public health because it includes several species that may cause diarrhoea. poultry and poultry products are known as important sources of human campylobacteriosis. in this study, during the autumn months of 2005, a total of 100 samples from poultry carcasses, representing 20 broiler flocks were obtained by rinse test, after the chilling stage ...